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[肺癌细胞系中热休克蛋白90（Hsp90）上调与顺铂耐药性之间的相关性]

[The correlation between the up-regulation of Hsp90 and drug resistance to cisplatin in lung cancer cell line].

作者信息

Liu Ting, Wang Xiaokun, Zhang Lichuan

机构信息

Department of Respiratory Medicine, the Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China.

出版信息

Zhongguo Fei Ai Za Zhi. 2011 Jun;14(6):472-7. doi: 10.3779/j.issn.1009-3419.2011.06.02.

DOI:10.3779/j.issn.1009-3419.2011.06.02
PMID:21645448
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5999893/
Abstract

BACKGROUND AND OBJECTIVE

Hsp90 is a major molecular chaperone, which overexpression is involved in oncogenesis, development and drug resistance in many human cancers. The aim of this study is to investigate the relationship between the GGA-induced overexpression of Hsp90 and chemoresistance to Cisplatin in SPCA-1 and H446 cell line.

METHODS

The protein expressions of Hsp90 induced by GGA at different concentrations were analyzed by Immunofluorescence and Western blotting. Cells survival to Cisplatin was determined using the MTT assays. The effect of Hsp90 expression on the drug resistance to Cisplatin in two Cell Lines was analyzed.

RESULTS

Compared with the respective control cells, Hsp90 expressions in both experimental cell lines were up-regulated obviously, exhibiting a dose-dependent manner to GGA. MTT assays revealed that the IC50s of cisplatin also showed a substantial elevation for the experimental cells of SPCA-1 and H446, and this elevation was also associated with GGA concerntration.

CONCLUSION

GGA is effective for the induction of Hsp90 in the SPCA-1 and H446 cell line. Up-regulated Hsp90 is associated with the chemoresistance to Cisplatin in SPCA-1and H446 cells.

摘要

背景与目的

热休克蛋白90（Hsp90）是一种主要的分子伴侣，其过表达参与多种人类癌症的发生、发展及耐药过程。本研究旨在探讨GGA诱导的Hsp90过表达与SPCA-1和H446细胞系对顺铂耐药性之间的关系。

方法

采用免疫荧光和蛋白质印迹法分析不同浓度GGA诱导的Hsp90蛋白表达。采用MTT法测定细胞对顺铂的存活率。分析Hsp90表达对两种细胞系顺铂耐药性的影响。

结果

与各自的对照细胞相比，两个实验细胞系中的Hsp90表达均明显上调，对GGA呈剂量依赖性。MTT分析显示，SPCA-1和H446实验细胞的顺铂半数抑制浓度（IC50）也显著升高，且这种升高也与GGA浓度相关。

结论

GGA能有效诱导SPCA-1和H446细胞系中的Hsp90表达。Hsp90表达上调与SPCA-1和H446细胞对顺铂的耐药性相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a99e/5999893/9f4517361533/zgfazz-14-6-472-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a99e/5999893/d671c483082a/zgfazz-14-6-472-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a99e/5999893/e51c9b5731b0/zgfazz-14-6-472-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a99e/5999893/7e2462c5cc99/zgfazz-14-6-472-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a99e/5999893/9f4517361533/zgfazz-14-6-472-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a99e/5999893/d671c483082a/zgfazz-14-6-472-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a99e/5999893/e51c9b5731b0/zgfazz-14-6-472-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a99e/5999893/7e2462c5cc99/zgfazz-14-6-472-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a99e/5999893/9f4517361533/zgfazz-14-6-472-4.jpg

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本文引用的文献

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Mol Pharmacol. 2007 Nov;72(5):1337-48. doi: 10.1124/mol.107.039164. Epub 2007 Aug 16.
2
Activation of Akt and ERK signalling pathways induced by etoposide confer chemoresistance in gastric cancer cells.依托泊苷诱导的Akt和ERK信号通路激活赋予胃癌细胞化学抗性。
Dig Liver Dis. 2006 May;38(5):310-8. doi: 10.1016/j.dld.2006.01.012. Epub 2006 Mar 9.
3
Discordant influence of amphotericin B on epirubicin cytotoxicity in primary hepatic malignant cells collected by a new primary culture technique.
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J Gastroenterol Hepatol. 2006 Feb;21(2):398-405. doi: 10.1111/j.1440-1746.2005.04026.x.
4
Geranylgeranylacetone protects against acetaminophen-induced hepatotoxicity by inducing heat shock protein 70.香叶基香叶基丙酮通过诱导热休克蛋白70来预防对乙酰氨基酚诱导的肝毒性。
Toxicology. 2006 Feb 15;219(1-3):187-96. doi: 10.1016/j.tox.2005.11.018. Epub 2005 Dec 27.
5
Pharmacological induction of heat-shock proteins alleviates polyglutamine-mediated motor neuron disease.热休克蛋白的药理学诱导可减轻多聚谷氨酰胺介导的运动神经元疾病。
Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16801-6. doi: 10.1073/pnas.0506249102. Epub 2005 Oct 31.
6
[Heat shock protein 90: novel target for cancer therapy].[热休克蛋白90：癌症治疗的新靶点]
Ai Zheng. 2004 Aug;23(8):968-74.
7
Inhibition of Hsp90: a new strategy for inhibiting protein kinases.热休克蛋白90（Hsp90）的抑制：一种抑制蛋白激酶的新策略。
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8
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10
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Dev Comp Immunol. 2003 Jun-Jul;27(6-7):449-64. doi: 10.1016/s0145-305x(02)00160-x.