High-tech Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
J Insect Sci. 2010;10:86. doi: 10.1673/031.010.8601.
The phylogenetic relationship between the whitefly Bemisia afer (Priesner & Hosny) (Hemiptera: Aleyrodidae) from China and other populations among the world were analyzed based on the mitochondrial cytochrome oxidase I (mtCOI) gene. Phylogenetic analysis of mtCOI sequences and those of reference B. afer sequences showed that the populations of the species could be separated into 5 clades (I-V). There were at least two clades of the species from China (IV and V). These data suggested that B. afer might be a species complex. The Chinese B. afer populations were most divergent with B. afer from the United Kingdom and African countries. The distance between the Chinese B. afer (IV and V) and clades I, II, and III is more than 32%, while the distance among clades I, II, III is lower than 7.7%. A new set of primers specific to B. afer was designed to amplify a region of approximately 400 bp to discriminate B. afer from other Bemisia species in China based on mtCOI sequences.
基于线粒体细胞色素氧化酶 I（mtCOI）基因，分析了来自中国的烟粉虱 Bemisia afer（Priesner & Hosny）（半翅目：粉虱科）与世界其他种群之间的系统发育关系。mtCOI 序列和参考的 B. afer 序列的系统发育分析表明，该物种的种群可以分为 5 个分支（I-V）。中国的该物种至少有两个分支（IV 和 V）。这些数据表明，B. afer 可能是一个物种复合体。来自中国的 B. afer 种群与来自英国和非洲国家的 B. afer 种群分歧最大。中国的 B. afer（IV 和 V）与分支 I、II 和 III 之间的距离超过 32%，而分支 I、II 和 III 之间的距离低于 7.7%。根据 mtCOI 序列，设计了一对新的特异性引物，用于扩增大约 400bp 的区域，以区分中国的 B. afer 与其他烟粉虱物种。
