Hu Jian, Zhang Xiaoyun, Jiang Zhilin, Zhang Feifei, Liu Yuanyuan, Li Zhan, Zhang Zhongkai
a Ministry of Agriculture Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation , Yunnan Provincial Key Lab of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences , Kunming , China.
b School of Biology and Chemistry, Pu'er University , Pu'er , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Apr;29(3):474-484. doi: 10.1080/24701394.2017.1307974. Epub 2017 Apr 1.
The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex and widely distributed throughout tropical and subtropical regions. To understand the B. tabaci cryptic species diversity in China more comprehensively, in the year 2014 and 2016, a large-scale sampling was conducted from the famous biodiversity hotspot of China, Yunnan province. Mitochondrial cytochrome oxidase I gene sequences were used to identify new putative cryptic species. Phylogenetic analyses were performed using Bayesian methods to evaluate the position of new cryptic species in the context of the B. tabaci diversity in Asia. Two new cryptic species, China 5 and Asia V were identified. In total, 19 B. tabaci cryptic species are present in China, two invasive (MED and MEAM1) and 17 indigenous. A new sibling species of B. tabaci was first defined and reported. Based on the mtCOI sequences and haplotype network analyses, the genetic diversity of MED was far higher than MEAM1. We confirmed the exotic MED was originated from the western Mediterranean regions and first invaded into Yunnan, China. The genetic structures of other four indigenous species (Asia I, Asia II 1, Asia II 6, and China 1) with relatively wide distribution ranges in China were also discussed.
烟粉虱Bemisia tabaci（Gennadius）（半翅目：粉虱科）是一个隐存种复合体，广泛分布于热带和亚热带地区。为了更全面地了解中国烟粉虱隐存种的多样性，在2014年和2016年，从中国著名的生物多样性热点地区云南省进行了大规模采样。利用线粒体细胞色素氧化酶I基因序列来鉴定新的假定隐存种。使用贝叶斯方法进行系统发育分析，以评估新隐存种在亚洲烟粉虱多样性背景下的位置。鉴定出两个新的隐存种，即中国5号和亚洲V型。中国共有19种烟粉虱隐存种，其中两种为入侵种（中东-地中海型和中东-亚洲1型），17种为本土种。首次定义并报道了烟粉虱的一个新姊妹种。基于线粒体细胞色素氧化酶I序列和单倍型网络分析，中东-地中海型的遗传多样性远高于中东-亚洲1型。我们证实外来的中东-地中海型起源于西地中海地区，首次入侵中国云南。还讨论了在中国分布范围相对较广的其他四个本土种（亚洲I型、亚洲II 1型、亚洲II 6型和中国1型）的遗传结构。
