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[维生素C对A549细胞增殖、凋亡及半胱天冬酶、生存素表达的影响]

[Effects of vitamin C on A549 cell proliferation, apoptosis and expressions of Caspase, Survivin].

作者信息

Zhai Pengyong, Zeng Jinrong, Tan Ning, Wang Jiying, Huang Lanzhen, She Weiwei

机构信息

Department of Respiritory, Affiliated Hospital of Guilin Medical University, Guilin 541000, China.

出版信息

Zhongguo Fei Ai Za Zhi. 2010 Feb;13(2):89-93. doi: 10.3779/j.issn.1009-3419.2010.02.01.

DOI:10.3779/j.issn.1009-3419.2010.02.01
PMID:20673497
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6000520/
Abstract

BACKGROUND AND OBJECTIVE

It was proven that Vitamin C could inhibit the growth of many types of tumors as an antioxidant. The aim of this study is to explore role of Vitamin C in proliferation and apoptosis of lung carcinoma cell line A549 and the underlying mechanism.

METHODS

A549 cells were cultured in vitro and incubated with Vitamin C. The cell viability was measured by growth curve and clonogentic assay. Flow cytometry was used to analyze cell cycle and detect apoptosis. The levels of expression of Caspase-3 mRNA and Survivin mRNA were detected by RT-PCR.

RESULTS

Vitamin C of 400 microg/mL, 4 mg/mL significantly inhibited the growth of A549 cell lines (P = 0.024, P = 0.015, respectively). Flow cytometry showed that the cells major stagnation stayed in the G0/G1 and S phase and the apoptotic rate increased with time prolonged. Vitamin C signifiantly up-regulated the expression of Caspase-3 mRNA, but had no effect on Survivin mRNA.

CONCLUSION

Vitamin C can inhibit the proliferation of A549, block A549 cells in G0/G1 and S phase, and induce apoptosis of A549 cells. Apotosis occurred by up-regulated the expression of Caspase-3.

摘要

背景与目的

业已证实，维生素C作为一种抗氧化剂可抑制多种肿瘤的生长。本研究旨在探讨维生素C在肺癌细胞系A549增殖和凋亡中的作用及其潜在机制。

方法

体外培养A549细胞，并与维生素C孵育。通过生长曲线和克隆形成试验检测细胞活力。采用流式细胞术分析细胞周期并检测细胞凋亡。通过逆转录-聚合酶链反应（RT-PCR）检测半胱天冬酶-3（Caspase-3）mRNA和生存素（Survivin）mRNA的表达水平。

结果

400μg/mL、4mg/mL的维生素C显著抑制A549细胞系的生长（分别为P = 0.024，P = 0.015）。流式细胞术显示，细胞主要停滞在G0/G1期和S期，且凋亡率随时间延长而增加。维生素C显著上调Caspase-3 mRNA的表达，但对Survivin mRNA无影响。

结论

维生素C可抑制A549细胞的增殖，使A549细胞阻滞于G0/G1期和S期，并诱导A549细胞凋亡。凋亡通过上调Caspase-3的表达而发生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/a14d70378e55/zgfazz-13-2-89-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/26df8e97b0f9/zgfazz-13-2-89-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/f9b5558d9c08/zgfazz-13-2-89-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/74d84128c3d8/zgfazz-13-2-89-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/727ce13e52f3/zgfazz-13-2-89-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/a14d70378e55/zgfazz-13-2-89-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/26df8e97b0f9/zgfazz-13-2-89-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/f9b5558d9c08/zgfazz-13-2-89-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/74d84128c3d8/zgfazz-13-2-89-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/727ce13e52f3/zgfazz-13-2-89-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ede/6000520/a14d70378e55/zgfazz-13-2-89-5.jpg

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Promyelocytic leukemia protein 4 induces apoptosis by inhibition of survivin expression.早幼粒细胞白血病蛋白4通过抑制生存素表达诱导细胞凋亡。
J Biol Chem. 2004 Jan 16;279(3):1838-44. doi: 10.1074/jbc.M310987200. Epub 2003 Nov 3.
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过氧化氢和维生素 C 对鸡胚成纤维细胞的凋亡作用：氧化还原状态与细胞程序性死亡。
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