血浆 Epstein-Barr 病毒载量测量作为 HIV 感染患者淋巴瘤的诊断标志物。

Plasma Epstein-Barr viral load measurement as a diagnostic marker of lymphoma in HIV-infected patients.

机构信息

Department of Hematology, Hospital Universitari Germans Trias i Pujol, Badalona, Universitat Autònoma de Barcelona, Spain.

出版信息

Med Clin (Barc). 2010 Oct 9;135(11):485-90. doi: 10.1016/j.medcli.2010.02.041. Epub 2010 Jul 31.

DOI:10.1016/j.medcli.2010.02.041

PMID:20673682

Abstract

BACKGROUND AND OBJECTIVES

To assess the use of the Epstein-Barr virus (EBV) viral load as a marker for lymphoma diagnosis in HIV-infected patients. We also aimed to identify the relationship between EBV viral load in plasma and the presence of EBV in lymphoma cells.

PATIENTS AND METHODS

Retrospective observational study of two HIV-infected populations: one of patients diagnosed with lymphoma and a control group. Thirty-nine patients with AIDS-related lymphoma (ARL) (32 non-Hodgkin's and 7 Hodgkin's lymphomas) and 134 HIV-positive individuals without neoplasia or opportunistic infections were studied. Blood samples were collected before lymphoma treatment in ARL patients. EBV viral load was measured in plasma by real-time quantitative PCR and the presence of EBV-EBER mRNA in lymphoma tumor was investigated by in situ hybridization.

RESULTS

Patients with ARL had higher EBV viral loads than those without lymphoma: 24,180.5 (±73,387.6)copies/mL versus 2.6 (±21.6)copies/mL (p<0.001). HIV-infected patients without lymphoma had negative or very low EBV load values. Among ARL patients, no correlation was found between EBV viral loads and CD4+ lymphocyte counts or between EBV and HIV RNA loads, or any other clinical or biological parameter. Cases with an EBV-EBER-positive lymphoma had higher EBV viral loads than those with EBER-negative tumors.

CONCLUSIONS

EBV viral load is a useful marker of lymphoma in HIV-infected patients, and may be a useful tool for early diagnosis and treatment.

摘要

背景与目的

评估 Epstein-Barr 病毒（EBV）病毒载量作为 HIV 感染患者淋巴瘤诊断的标志物。我们还旨在确定血浆中 EBV 病毒载量与淋巴瘤细胞中 EBV 存在之间的关系。

患者与方法

对两个 HIV 感染人群进行回顾性观察性研究：一组为诊断为淋巴瘤的患者，另一组为对照组。研究了 39 例 AIDS 相关淋巴瘤（ARL）患者（32 例非霍奇金淋巴瘤和 7 例霍奇金淋巴瘤）和 134 例无肿瘤或机会性感染的 HIV 阳性个体。在 ARL 患者接受淋巴瘤治疗前采集血液样本。通过实时定量 PCR 测量血浆中的 EBV 病毒载量，并通过原位杂交研究淋巴瘤肿瘤中 EBV-EBER mRNA 的存在。

结果

ARL 患者的 EBV 病毒载量高于无淋巴瘤患者：24180.5（±73387.6）拷贝/mL 与 2.6（±21.6）拷贝/mL（p<0.001）。无淋巴瘤的 HIV 感染患者的 EBV 载量为阴性或极低。在 ARL 患者中，未发现 EBV 病毒载量与 CD4+淋巴细胞计数之间或 EBV 与 HIV RNA 载量之间存在相关性，或与任何其他临床或生物学参数相关。EBV-EBER 阳性淋巴瘤病例的 EBV 病毒载量高于 EBER 阴性肿瘤病例。