Bonnet Fabrice, Jouvencel Anne-Christine, Parrens Marie, Leon Magali Joblon, Cotto Emmanuelle, Garrigue Isabelle, Morlat Philippe, Beylot Jacques, Fleury Hervé, Lafon Marie-Edith
Service de Médecine Interne et Maladies Infectieuses, Hôpital Saint-André, 1 rue Jean Burguet, 33075 Bordeaux Cedex, France.
J Clin Virol. 2006 Aug;36(4):258-63. doi: 10.1016/j.jcv.2006.04.005. Epub 2006 Jun 9.
Epstein-Barr virus (EBV) may be causally associated with non-Hodgkin Lymphoma (NHL) in HIV-infected patients.
To compare EBV load in whole blood in AIDS-NHL patients, HIV non-AIDS patients and non-HIV-infected persons, and to prospectively measure EBV load in whole blood in AIDS-NHL patients.
Longitudinal and prospective study.
We observed no statistical difference in EBV load between AIDS-NHL (3.69log(10) copies/mL [interquartile range (IQR): 2.89-4.27]) and HIV non-AIDS patients (3.08log(10) copies/mL [IQR: 1.29-3.57]) but AIDS-NHL patients had significantly higher EBV loads than HIV-negative controls (1.19log(10) copies/mL [IQR: 0.00-3.29]). We noticed an inverse correlation between CD4+ lymphocytes count and EBV load in patients with AIDS-NHL (r(2)=0.41, P=0.01). In the longitudinal study, the mean EBV load three months after NHL diagnosis decreased significantly (mean difference=-1.69log(10) copies/mL [95% confidence interval: -0.32; -3.04]; P=0.03) under chemotherapy but was still elevated in patients with relapses or no response to chemotherapy.
Although EBV load seems a suboptimal marker for the diagnosis of AIDS-NHL, we observed a significant decrease of EBV load in patients treated with chemotherapy and a strong association between NHL outcome and EBV load in whole blood.
爱泼斯坦-巴尔病毒（EBV）可能与HIV感染患者的非霍奇金淋巴瘤（NHL）存在因果关联。
比较艾滋病相关NHL患者、HIV非艾滋病患者和未感染HIV者全血中的EBV载量，并前瞻性地检测艾滋病相关NHL患者全血中的EBV载量。
纵向前瞻性研究。
我们观察到，艾滋病相关NHL患者（3.69log₁₀拷贝/毫升[四分位间距（IQR）：2.89 - 4.27]）和HIV非艾滋病患者（3.08log₁₀拷贝/毫升[IQR：1.29 - 3.57]）之间的EBV载量无统计学差异，但艾滋病相关NHL患者的EBV载量显著高于HIV阴性对照（1.19log₁₀拷贝/毫升[IQR：0.00 - 3.29]）。我们注意到艾滋病相关NHL患者的CD4⁺淋巴细胞计数与EBV载量呈负相关（r² = 0.41，P = 0.01）。在纵向研究中，NHL诊断后三个月，化疗后患者的平均EBV载量显著下降（平均差值 = -1.69log₁₀拷贝/毫升[95%置信区间：-0.32；-3.04]；P = 0.03），但复发或对化疗无反应的患者其EBV载量仍升高。
尽管EBV载量似乎不是诊断艾滋病相关NHL的理想标志物，但我们观察到化疗患者的EBV载量显著下降，且NHL预后与全血EBV载量之间存在密切关联。
