Bioenvironmental Engineering Research Unit, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia.
Bioresour Technol. 2010 Dec;101(23):9241-7. doi: 10.1016/j.biortech.2010.07.024. Epub 2010 Jul 13.
This paper introduces sludge palm oil (SPO) as a novel substrate for biosurfactant production by liquid state fermentation. Potential strains of microorganism were isolated from various hydrocarbon-based sources at palm oil mill and screened for biosurfactant production with the help of drop collapse method and surface tension activity. Out of 22 isolates of microorganism, the strain S02 showed the highest bacterial growth with a surface tension of 36.2 mN/m and was therefore, selected as a potential biosurfactant producing microorganism. Plackett-Burman experimental design was employed to determine the important nutritional requirement for biosurfactant production by the selected strain under controlled conditions. Six out of 11 factors of the production medium were found to significantly affect the biosurfactant production. K(2)HPO(4) had a direct proportional correlation with the biosurfactant production while sucrose, glucose, FeSO(4), MgSO(4), and NaNO(3) showed inversely proportional relationship with biosurfactant production in the selected experimental range.
本文介绍了一种新型的生物表面活性剂生产底物——污泥棕榈油（SPO），它可通过液态发酵来制取。我们从棕榈油厂的各种碳氢化合物源中分离出潜在的微生物菌株，并借助滴陷法和表面张力活性对其进行生物表面活性剂生产筛选。在 22 株微生物分离株中，菌株 S02 的细菌生长最高，表面张力为 36.2 mN/m，因此被选为具有生产生物表面活性剂的潜力的微生物。我们采用 Plackett-Burman 实验设计来确定在控制条件下，所选菌株生产生物表面活性剂的重要营养需求。在生产培养基的 11 个因素中，有 6 个因素被发现对生物表面活性剂的生产有显著影响。K2HPO4 与生物表面活性剂的生产呈直接比例关系，而蔗糖、葡萄糖、FeSO4、MgSO4 和 NaNO3 在所选实验范围内与生物表面活性剂的生产呈反比关系。
