Jamal Parveen, Mir Shajrat, Alam Md Zahangir, Wan Nawawi Wan M Fazli
Bioenvironmental Engineering Research Centre (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia.
J Oleo Sci. 2014;63(8):795-804. doi: 10.5650/jos.ess13181. Epub 2014 Jul 8.
Biosurfactants are surface-active compounds produced by different microorganisms. The aim of this study was to introduce palm kernel cake (PKC) as a novel substrate for biosurfactant production using a potent bacterial strain under liquid state fermentation. This study was primarily based on the isolation and identification of biosurfactant-producing bacteria that could utilize palm kernel cake as a new major substrate. Potential bacterial strains were isolated from degraded PKC and screened for biosurfactant production with the help of the drop collapse assay and by analyzing the surface tension activity. From the screened isolates, a new strain, SM03, showed the best and most consistent results, and was therefore selected as the most potent biosurfactant-producing bacterial strain. The new strain was identified as Providencia alcalifaciens SM03 using the Gen III MicroPlate Biolog Microbial Identification System. The yield of the produced biosurfactant was 8.3 g/L.
生物表面活性剂是由不同微生物产生的表面活性化合物。本研究的目的是引入棕榈仁粕（PKC）作为一种新型底物，用于在液态发酵条件下利用一种高效细菌菌株生产生物表面活性剂。本研究主要基于对能够利用棕榈仁粕作为新主要底物的产生物表面活性剂细菌的分离和鉴定。从降解的棕榈仁粕中分离出潜在的细菌菌株，并借助液滴塌陷试验和分析表面张力活性来筛选生物表面活性剂的产生情况。在筛选出的分离株中，新菌株SM03表现出最佳且最稳定的结果，因此被选为最有效的产生物表面活性剂细菌菌株。使用Gen III微孔板Biolog微生物鉴定系统将该新菌株鉴定为产碱普罗威登斯菌SM03。所产生的生物表面活性剂的产量为8.3 g/L。
