Nasir Mohd Shafiq, Mohd Yahya Ahmad Ramli, Md Noh Nur Asshifa
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM Pulau Pinang, Malaysia.
Trop Life Sci Res. 2024 Mar;35(1):33-47. doi: 10.21315/tlsr2024.35.1.3. Epub 2024 Mar 30.
Rhamnolipid has gained much attention in various fields owing to its distinctive functional properties compared to conventional chemical surfactants, which are mostly derived from petroleum feedstock. Production cost is one of the main challenges in rhamnolipid production, particularly when using refined substrates. One possible solution is to use agro-industrial wastes as substrates for rhamnolipid production. This is a promising strategy due to their abundance and commercially low value, while simultaneously alleviating an agro-industrial waste management problem in the environment. This study aims to evaluate agro-industrial wastes from local crops as possible low-cost alternative substrates for rhamnolipid production by a local isolate, USM-AR2. Various liquid wastes, namely sugarcane molasses, rice washing water, overly mature coconut (OMC) water, empty fruit bunch (EFB) steam effluent, palm sludge oil (PSO) and palm oil mill effluent (POME) were screened as the main carbon source supplementing mineral salt medium (MSM) in the fermentation of USM-AR2. Batch fermentation was carried out in a shake flask system, agitated at 200 rpm and incubated at room temperature, 27 ± 2°C for 120 h. Among the substrates tested, PSO exhibited the highest biomass at 20.78 g/L and rhamnolipid production at 1.07 g/L. This study has shown the potential of agro-industrial wastes in Malaysia as an alternative resource for rhamnolipid production, transforming them into value added products, while reducing the amount of wastes discharged into the environment.
与大多源自石油原料的传统化学表面活性剂相比，鼠李糖脂因其独特的功能特性而在各个领域备受关注。生产成本是鼠李糖脂生产中的主要挑战之一，尤其是在使用精制底物时。一种可能的解决方案是使用农业工业废弃物作为鼠李糖脂生产的底物。这是一种很有前景的策略，因为它们储量丰富且商业价值低，同时还能缓解环境中的农业工业废弃物管理问题。本研究旨在评估当地作物产生的农业工业废弃物作为当地菌株USM - AR2生产鼠李糖脂的低成本替代底物的可能性。筛选了各种液体废弃物，即甘蔗 molasses、洗米水、过度成熟椰子（OMC）水、空果串（EFB）蒸汽流出物、棕榈污泥油（PSO）和棕榈油厂废水（POME），作为在USM - AR2发酵过程中补充矿物盐培养基（MSM）的主要碳源。分批发酵在摇瓶系统中进行，以200 rpm搅拌，并在室温27±2°C下孵育120小时。在所测试的底物中，PSO的生物量最高，为20.78 g/L，鼠李糖脂产量为1.07 g/L。本研究表明，马来西亚的农业工业废弃物有潜力作为鼠李糖脂生产的替代资源，将它们转化为增值产品，同时减少排放到环境中的废弃物量。
