Microbial Technology Laboratory, Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, India.
Bioresour Technol. 2010 Dec;101(23):9248-53. doi: 10.1016/j.biortech.2010.07.042. Epub 2010 Jul 14.
Degradation by white rot fungi has the potential to increase digestibility of wheat straw and thus improve its value as animal feed. To optimize conditions for production of lignocellulolytic enzymes by Phlebia floridensis during solid state fermentation of wheat straw along with enhancement of in vitro digestibility, a response surface methodology (RSM) based experiment was designed. Effect of moisture content, inorganic nitrogen source (NH(4)Cl) and malt extract on lignocellulolytic enzymes, changes in chemical constituents and digestibility of wheat straw was evaluated. With increase in moisture content, laccase production increased up to 34-fold, while Manganese peroxidase was optimally produced in the presence of almost equal amount (50-55 mg/g of WS) of NH(4)Cl and malt extract. These supplements also significantly (p<0.05) enhanced the production of CMCase and xylanase. In vitro digestibility was increased by almost 50% with a loss of 27.6% and 14.6% in lignin and total organic matter, respectively. The present findings revealed P. floridensis to be an efficient organism for lignocellulolytic enzymes production and simultaneous enhancement in in vitro digestibility of wheat straw.
白腐真菌的降解作用有可能提高小麦秸秆的消化率，从而提高其作为动物饲料的价值。为了优化白腐菌在固态发酵小麦秸秆过程中木质纤维素酶的生产条件，并提高体外消化率，采用响应面法（RSM）设计了实验。评估了水分含量、无机氮源（氯化铵）和麦芽提取物对木质纤维素酶、小麦秸秆化学成分变化和消化率的影响。随着水分含量的增加，漆酶的产量增加了 34 倍，而锰过氧化物酶在存在等量（50-55mg/gWS）氯化铵和麦芽提取物的情况下得到了最佳生产。这些补充剂还显著（p<0.05）提高了CMC 酶和木聚糖酶的产量。体外消化率提高了近 50%，木质素和总有机物质分别损失了 27.6%和 14.6%。本研究结果表明，白腐菌是一种有效的木质纤维素酶生产和同时提高小麦秸秆体外消化率的生物体。
