Microbial Technology Laboratory, Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, India.
Bioresour Technol. 2011 Sep;102(17):8085-91. doi: 10.1016/j.biortech.2011.06.007. Epub 2011 Jun 13.
Bioprocessing of wheat straw was carried out by Phlebia brevispora under solid state conditions. Effect of different supplements on lignocellulolytic enzymes production, degradation of straw cell wall fibers and its resultant effect on nutritional quality of wheat straw were studied. Ammonium chloride and malt extract were more effective in terms of ligninolysis and enhanced in vitro digestibility. The concentration of the selected supplements and the moisture content was worked out using response surface methodology in order to minimize the loss in total organic matter so as to selectively degrade lignin. The experiment was scaled up to batches of 200 g under optimized conditions and the degraded substrate was analyzed for its biochemical properties. P. brevispora degraded 290 g/kg of lignin and enhanced the in vitro digestibility from 150 to 268 g/kg (78%). Crude protein, amino acids, total phenolic contents and antioxidant properties were significantly higher in degraded straw.
采用栓菌在固态条件下对麦秸进行生物加工。研究了不同辅料对木质纤维素酶产生、麦秸细胞壁纤维降解及其对麦秸营养品质的影响。氯化铵和麦芽提取物在木质素降解方面更有效,并且提高了体外消化率。通过响应面法确定了所选辅料的浓度和水分含量,以尽量减少总有机物质的损失,从而有选择地降解木质素。在优化条件下将实验扩大到 200 克批次,并对降解的基质进行了生化特性分析。栓菌降解了 290 克/千克的木质素,使体外消化率从 150 克/千克提高到 268 克/千克(78%)。降解后的麦秸中的粗蛋白、氨基酸、总酚含量和抗氧化性能显著提高。