Schimmer O, Kühne I
Institut für Botanik und Pharmazeutische Biologie, Universität Erlangen-Nürnberg, F.R.G.
Mutat Res. 1991 Jul;249(1):105-10. doi: 10.1016/0027-5107(91)90136-c.
Seven naturally occurring furoquinoline alkaloids were investigated for their photobiological activity using arg-1 cells of Chlamydomonas reinhardtii. UV-A-mediated toxicity of the compounds was calculated from the colony-forming ability of the treated cells. The UV-A-mediated mutagenicity was measured by counting the number of Arg+ revertants induced by the treatment. Dictamnine was found to be the strongest mutagen as well as the most toxic compound of the group. The mutagenic activities were measured as mutation frequencies at equal substance concentration and ranked in the following order: An increase in the number of substituents on the lateral aromatic nucleus greatly decreased the photomutagenicity. Except for evolitrine, a similar ranking order was found as reported for the dark mutagenicity of these compounds in Salmonella typhimurium strain TA98. Based on the result that furoquinolines are able to intercalate into DNA, we assume that the different mutagenic potencies may reflect differences in the geometry of the intercalation complex, which is important for the subsequent photochemical reaction.
利用莱茵衣藻的arg-1细胞，对七种天然存在的呋喃喹啉生物碱的光生物学活性进行了研究。根据处理后细胞的集落形成能力计算化合物的UV-A介导毒性。通过计数处理诱导的Arg+回复突变体数量来测量UV-A介导的诱变性。发现白藓碱是该组中最强的诱变剂和毒性最大的化合物。诱变活性以同等物质浓度下突变频率来衡量，并按以下顺序排列：芳环侧链上取代基数量的增加极大地降低了光致诱变性。除了吴茱萸次碱，发现其与这些化合物在鼠伤寒沙门氏菌TA98菌株中的暗诱变性报道的排列顺序相似。基于呋喃喹啉能够插入DNA的结果，我们推测不同的诱变能力可能反映了插入复合物几何形状的差异，这对随后的光化学反应很重要。
