Mizuta M, Kanamori H
Mutat Res. 1985 Dec;144(4):221-5. doi: 10.1016/0165-7992(85)90054-5.
A methanol extract of Dictamni Radicis Cortex exhibited a mutagenic effect on Salmonella typhimurium TA100 and TA98 with S9 mix. Two mutagenic compounds in Dictamni Radicis Cortex were isolated on a Sephadex LH 20 column and silica gel column chromatography and by preparative TLC. These were identified as dictamnine and gamma-fagarine by UV, EI-Mass, 1H-NMR. Dictamnine and gamma-fagarine were mutagenic in strain TA100 and TA98 with S9 mix. The dose-response curves were linear in the range 10-40 micrograms. Dictamnine and gamma-fagarine had specific activities (His+/microgram) of about 50-70 revertant colonies in strain TA100, while in strain TA98 there were about 30-50 revertant colonies.
白鲜皮甲醇提取物在有S9混合液存在的情况下，对鼠伤寒沙门氏菌TA100和TA98表现出诱变作用。通过葡聚糖凝胶LH 20柱、硅胶柱色谱法以及制备薄层色谱法，从白鲜皮中分离出两种诱变化合物。通过紫外光谱、电子轰击质谱、氢核磁共振谱将它们鉴定为白鲜碱和γ-崖椒碱。白鲜碱和γ-崖椒碱在有S9混合液存在的情况下，对TA100和TA98菌株具有诱变作用。在10 - 40微克的范围内，剂量反应曲线呈线性。白鲜碱和γ-崖椒碱在TA100菌株中的比活性（His + /微克）约为50 - 70个回复突变菌落，而在TA98菌株中约为30 - 50个回复突变菌落。
