Bossowski A, Czarnocka B, Bardadin K, Moniuszko A, Łyczkowska A, Czerwinska J, Dadan J, Bossowska A
Department of Paediatrics, Endocrinology, Diabetology with Cardiology Division, Medical University in Bialystok, Poland.
Folia Histochem Cytobiol. 2010 Jan;48(2):178-84. doi: 10.2478/v10042-010-0022-2.
The aim of this study was to estimate sodium iodide symporter (NIS) and thyroid peroxidase (TPO) expression in thyrocytes from patients with GD and no-toxic multinodular goitre (NTMG) in relationship with apoptotic (TIAR and TIA-1) markers. The investigation was performed on thyroid cells isolated from postoperation thyroid tissues from 15 patients aged 12-21 years old with GD and 15 cases aged 13-21 years old with NTMG. Detection of NIS and TPO was performed by immunohistochemistry. Analysis of apoptotic markers in thyroid tissues was performed using antibodies to TIAR and TIA-1 by Western Blot and immunohistochemistry. Identification of proapoptotic TIAR and TIA-1 molecules in the thyroid tissues revealed a higher expression of both proteins in patients with Graves' disease (+++; +, respectively) in comparison to patients with NTNG (+; 0). In addition, TIAR expression was detected in three bands [p50, p42, p38 (kDa)] and TIA-1 in two bands [p22, p17 (kDa)]. using Western Blot test in patients with thyroid autoimmune diseases. In patients with NTNG expression of both apoptotic proteins was lower and identified in single bands: 42 (kDa) for TIAR and 17 (kDa) for TIA-1. The analysis of expression of NIS and TPO in thyroid follicular cells was higher in patients with Graves' disease in compared to their detection in patients with NTMG. In addition, degree of thyroid antigen expression positive correlated with amount of proapoptotic markers (TIAR, p<0.001; TIA-1, p<0.025 for NIS; TIAR, p<0.012 for TPO). We conclude that elevated expression of NIS and TPO in Graves' disease is associated with higher stimulation and activation of apoptosis in thyroid follicular cells during autoimmune process.
本研究旨在评估Graves病（GD）和非毒性多结节性甲状腺肿（NTMG）患者甲状腺细胞中碘化钠转运体（NIS）和甲状腺过氧化物酶（TPO）的表达，并分析其与凋亡标志物（TIAR和TIA-1）的关系。研究对象为15例年龄在12 - 21岁的GD患者和15例年龄在13 - 21岁的NTMG患者术后甲状腺组织分离出的甲状腺细胞。采用免疫组织化学法检测NIS和TPO。通过蛋白质免疫印迹法和免疫组织化学法，使用针对TIAR和TIA-1的抗体分析甲状腺组织中的凋亡标志物。在甲状腺组织中鉴定促凋亡TIAR和TIA-1分子发现，与NTNG患者（分别为+；0）相比，Graves病患者中这两种蛋白的表达更高（分别为+++；+）。此外，在甲状腺自身免疫性疾病患者中，通过蛋白质免疫印迹法检测发现TIAR表达于三条条带[p50、p42、p38（kDa）]，TIA-1表达于两条条带[p22、p17（kDa）]。在NTNG患者中，两种凋亡蛋白的表达较低，且TIAR在42（kDa）单一条带中被鉴定，TIA-1在17（kDa）单一条带中被鉴定。与NTMG患者相比，Graves病患者甲状腺滤泡细胞中NIS和TPO的表达分析结果更高。此外，甲状腺抗原表达程度与促凋亡标志物的量呈正相关（NIS的TIAR，p<0.001；TIA-1，p<0.025；TPO的TIAR，p<0.012）。我们得出结论，Graves病中NIS和TPO表达升高与自身免疫过程中甲状腺滤泡细胞凋亡的更高刺激和激活有关。
