Quemelo Paulo Roberto Veiga, Peres Luiz Cesar
Department of Physiotherapy, University of Franca, Franca - São Paulo, Brazil.
Acta Cir Bras. 2010 Aug;25(4):328-31. doi: 10.1590/s0102-86502010000400005.
To investigate the proliferation and neuronal death in brain tissue heterotopia in the lung in an experimental model during both fetal and neonatal periods.
Twenty four pregnant female Swiss mice were used to induce brain tissue heterotopia on the 15th gestational day. Briefly, the brain of one fetus of each dam was extracted, disaggregated and injected into the right hemithorax of siblings. Six of these fetuses with pulmonary brain tissue implantation (PBI) were collected on the 18th gestational day (group E18) and six other on the 8th postnatal day (group P8). Immunohistochemical staining for PCNA and Bcl2 were used to assess proliferation and cell death.
PCNA Labelling Index (LI) in heterotopic brain tissue was greater in fetal than postnatal period (E18 > P8) (p<0.05) and the immunostaining with Bcl2 antibody did not show difference.
Cell proliferation is maintained in brain tissue heterotopia, although apoptosis is also observed.
在一个实验模型中研究胎儿期和新生儿期肺组织中脑组织异位的增殖和神经元死亡情况。
24只怀孕的雌性瑞士小鼠在妊娠第15天用于诱导脑组织异位。简要来说，从每只母鼠的一个胎儿提取大脑，解离后注入同窝小鼠的右半胸。其中6只植入肺脑组织（PBI）的胎儿在妊娠第18天收集（E18组），另外6只在出生后第8天收集（P8组）。使用PCNA和Bcl2免疫组织化学染色评估增殖和细胞死亡情况。
异位脑组织中的PCNA标记指数（LI）在胎儿期高于出生后（E18＞P8）（p<0.05），并且用Bcl2抗体进行的免疫染色未显示出差异。
尽管也观察到细胞凋亡，但脑组织异位中细胞增殖仍持续存在。
