[广西巴马老年人群长寿相关线粒体基因组变异研究]
[Study on the longevity related mitochondrial genome variation in Bama elderly population in Guangxi province].
作者信息
Lv Ze-ping, Zheng Chen-guang, Kong Fang, Feng Jie, Jiang Wen-yu, Hu Cai-you, Li Hua, Lv Yuan, Zhang Gen-fa, Yang Ze
机构信息
Guangxi Jiangbin Hospital, Nanning, Guangxi, 530021 PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Aug;27(4):423-7. doi: 10.3760/cma.j.issn.1003-9406.2010.04.014.
OBJECTIVE
To investigate the human mitochondrial DNA (mtDNA) variations associated with longevity in Bama elderly population from Guangxi.
METHODS
Mitochondrial genome of 20 individuals over 96 years of age was sequenced, and seven target single nucleotide polymorphism (SNPs) were observed by comparing with the standard rCRS sequence, and two were tested by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in a larger population including 208 individuals of 90-113 years old, and 586 unrelated control individuals from Guangxi.
RESULTS
The 4824G frequency of the mtDNA4824A/G locus increased with age both in the long-lived elderly and in controls. And it was significantly higher in controls than that in long-lived population (P<0.05).
CONCLUSION
The mtDNA4824 A/G is not only an age-related locus, its mutation is also negatively correlated with longevity.
目的
研究广西巴马老年人群中与长寿相关的人类线粒体DNA（mtDNA）变异。
方法
对20名96岁以上个体的线粒体基因组进行测序，与标准rCRS序列比较观察到7个目标单核苷酸多态性（SNP），并在包括208名90 - 113岁个体和586名来自广西的无关对照个体的更大群体中通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测了2个。
结果
mtDNA4824A/G位点的4824G频率在长寿老年人和对照人群中均随年龄增加。且对照人群中的该频率显著高于长寿人群（P<0.05）。
结论
mtDNA4824 A/G不仅是一个与年龄相关的位点，其突变还与长寿呈负相关。
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