GI Motility Program, Division of Gastroenterology, Cedars-Sinai Medical Center, 8730 Alden Drive, Los Angeles, CA 90048, USA.
World J Gastroenterol. 2010 Aug 7;16(29):3680-6. doi: 10.3748/wjg.v16.i29.3680.
To investigate the interstitial cells of Cajal (ICC) number using a new rat model.
Sprague-Dawley rats were assigned to two groups. The first group received gavage with Campylobacter jejuni (C. jejuni) 81-176. The second group was gavaged with placebo. Three months after clearance of Campylobacter from the stool, precise segments of duodenum, jejunum, and ileum were ligated in self-contained loops of bowel that were preserved in anaerobic bags. Deep muscular plexus ICC (DMP-ICC) were quantified by two blinded readers assessing the tissue in a random, coded order. The number of ICC per villus was compared among controls, Campylobacter recovered rats without small intestinal bacterial overgrowth (SIBO), and Campylobacter recovered rats with SIBO.
Three months after recovery, 27% of rats gavaged with C. jejuni had SIBO. The rats with SIBO had a lower number of DMP-ICC than controls in the jejunum and ileum. Additionally there appeared to be a density threshold of 0.12 DMP-ICC/villus that was associated with SIBO. If ileal density of DMP-ICC was < 0.12 ICC/villus, 54% of rats had SIBO compared to 9% among ileal sections with > 0.12 (P < 0.05). If the density of ICC was < 0.12 DMP-ICC/villus in more than one location of the bowel, 88% of these had SIBO compared to 6% in those who did not (P < 0.001).
In this post-infectious rat model, the development of SIBO appears to be associated with a reduction in DMP-ICC. Further study of this rat model might help understand the pathophysiology of post-infectious irritable bowel syndrome.
利用一种新的大鼠模型研究 Cajal 间质细胞（ICC）的数量。
将 Sprague-Dawley 大鼠分为两组。第一组用空肠弯曲菌（C. jejuni）81-176 灌胃。第二组用安慰剂灌胃。粪便中弯曲菌清除 3 个月后，将精确的十二指肠、空肠和回肠段结扎成独立的肠襻，保存在厌氧袋中。通过两位盲法读者评估随机、编码的组织，对深肌丛 ICC（DMP-ICC）进行定量。将对照组、未发生小肠细菌过度生长（SIBO）的弯曲菌恢复大鼠和发生 SIBO 的弯曲菌恢复大鼠的每绒毛 ICC 数量进行比较。
在恢复后 3 个月，27%用 C. jejuni 灌胃的大鼠发生 SIBO。与对照组相比，发生 SIBO 的大鼠空肠和回肠的 DMP-ICC 数量较少。此外，似乎存在一个与 SIBO 相关的 0.12 DMP-ICC/绒毛密度阈值。如果回肠 DMP-ICC 密度<0.12 ICC/绒毛，则 54%的大鼠发生 SIBO，而>0.12 ICC/绒毛的回肠节段中 SIBO 发生率为 9%（P<0.05）。如果肠道的 ICC 密度<0.12 DMP-ICC/绒毛的部位多于一处，则 88%的大鼠发生 SIBO，而未发生 SIBO 的大鼠为 6%（P<0.001）。
在这种感染后大鼠模型中，SIBO 的发生似乎与 DMP-ICC 的减少有关。对这种大鼠模型的进一步研究可能有助于了解感染后肠易激综合征的病理生理学。
