[采用定量实时PCR检测hTERT mRNA在Anip973及Anip973/NVB细胞中的表达]
[Expression of hTERT mRNA in Anip973 and Anip973/NVB cell detected by quantitative real-time PCR].
作者信息
Chen Gongyan, Guo Shuying, Liu Jingjing
机构信息
Department of Medical Oncology, Tumor Hospital of Harbin Medical University, Harbin 150040, China.
出版信息
Zhongguo Fei Ai Za Zhi. 2010 Apr;13(4):297-300. doi: 10.3779/j.issn.1009-3419.2010.04.05.
BACKGROUND AND OBJECTIVE
Human telomerase reverse transcriptase is the catalytic subunit of telomerase, and its activity is correlated with cell's sensitivity to chemotherapy. The aim of this study is to investigate the differential expression of human telomerase reverse transcriptase (hTERT) mRNA in human lung adenocarcinoma cell line Anip973 and Anip973/NVB, and to observe the correlation between hTERT mRNA and drug-resistance.
METHODS
The real-time fluorescence quantitative RT-PCR was used to detect the change of hTERT mRNA in human lung adenocarcinoma drug-resistant cell Anip973/NVB and parental cell Anip973 treated by NVB.
RESULTS
In the control group, the expression of hTERT mRNA showed no significant difference between drug-resistant cell Anip973/NVB and parental cell Anip973. After been treated by NVB, the expression of hTERT mRNA in parental cell was significantly decreased (P < 0.01), and drug-resistant cell Anip973/ NVB had no evidently variant (P > 0.05). The down-regulated hTERT mRNA in Anip973 cell was higher than that in Anip973/ NVB cell.
CONCLUSION
Telomerase correlates with the drug-resistant cell A973/NVB, and telomerase may be a new target for multi-drug resistant inversion.
背景与目的
人端粒酶逆转录酶是端粒酶的催化亚基，其活性与细胞对化疗的敏感性相关。本研究旨在探讨人端粒酶逆转录酶（hTERT）mRNA在人肺腺癌细胞系Anip973及Anip973/NVB中的差异表达，并观察hTERT mRNA与耐药性之间的相关性。
方法
采用实时荧光定量RT-PCR检测长春瑞滨处理后人肺腺癌耐药细胞Anip973/NVB及亲本细胞Anip973中hTERT mRNA的变化。
结果
对照组中，耐药细胞Anip973/NVB与亲本细胞Anip973的hTERT mRNA表达无显著差异。经长春瑞滨处理后，亲本细胞中hTERT mRNA表达显著降低（P<0.01），而耐药细胞Anip973/NVB无明显变化（P>0.05）。Anip973细胞中hTERT mRNA下调程度高于Anip973/NVB细胞。
结论
端粒酶与耐药细胞A973/NVB相关，端粒酶可能是多药耐药逆转的新靶点。
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