Rahman M M, Rahman M M, Akhter S, Jamal M A H M, Pandeya D R, Haque M A, Alam M F, Rahman A
Department of Microbiology and Immunology, Chonbuk National University Medical School, Jeonju 561-712, Republic of Korea.
Nepal Med Coll J. 2010 Mar;12(1):12-9.
The aims of this study were to determine the total population of coliform bacteria in the samples collected from diarrhea associated patients from the local area of Bangladesh and to examine the antibacterial efficacy of leaf extracts of Moringa oleifera (Moringaceae) against the isolated coliform bacteria. The coliform bacteria detected in these samples by some microbial-biochemical tests such as Escherichia coli, Shigella dysenteriae, Salmonella sp., Enterobacter sp., Klebsiella pneumoniae and Serratia marcescens. The total isolation rate of coliform bacterial species was ranged from 38.01-3.51%. At the concentration of 300 ig/disc, the organic extracts of hexane, chloroform, ethyl acetate and methanol extracts of Moringa oleifera leaf exhibited a remarkable antibacterial effect against all the tested bacterial pathogens. The zones of inhibition against all the tested bacterial pathogens were found in the range of 8.0 to 23.2 mm, along with their respective minimum inhibitory concentration (MIC) values ranging from 62.5-1000 ig/mL. The results obtained in this study suggest that the extracts from Moringa oleifera leaf can be a source of natural antimicrobials with potential applications in pharmaceutical industry to control coliform bacteria.
本研究的目的是确定从孟加拉国当地腹泻相关患者采集的样本中大肠菌群细菌的总数，并检测辣木（辣木科）叶提取物对分离出的大肠菌群细菌的抗菌效果。通过一些微生物生化试验在这些样本中检测到大肠菌群细菌，如大肠杆菌、痢疾志贺氏菌、沙门氏菌属、肠杆菌属、肺炎克雷伯菌和粘质沙雷氏菌。大肠菌群细菌物种的总分离率在38.01%-3.51%之间。在300μg/圆盘的浓度下，辣木叶的己烷、氯仿、乙酸乙酯和甲醇提取物的有机提取物对所有测试的细菌病原体均表现出显著的抗菌作用。对所有测试细菌病原体的抑菌圈在8.0至23.2mm范围内，其各自的最低抑菌浓度（MIC）值在62.5-1000μg/mL之间。本研究获得的结果表明，辣木叶提取物可以成为天然抗菌剂的来源，在制药工业中具有控制大肠菌群细菌的潜在应用价值。
