Zheng Dejie, Hua Feng, Mei Chaorong, Wan Haisu, Zhou Qinghua
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Zhongguo Fei Ai Za Zhi. 2010 May;13(5):506-10. doi: 10.3779/j.issn.1009-3419.2010.05.23.
Glutathione S-transferase M1 (GSTM1) is an important phase II metabolic enzyme gene which involves metabolism of various carcinogens in human body. Many studies showed that GSTM1 genetic polymorphism was associated with lung cancer risk. The aim of this study is to investigate the relationship between GSTM1 genetic polymorphism and lung cancer risk among Han nationality population in Tianjin district.
GSTM1 genetic polymorphism was detected by melting curve analysis of SYBR green I real-time PCR assay. Two hundred and sixty-five histological confirmed lung cancer patients and 307 health controls were recruited in this case-control study and the relationship between GSTM1 genetic polymorphism and lung cancer riskwas investigated.
(1) The frequency of the GSTMI(-) in lung cancer and control groups was 56.6% and 57.0% respectively, and no significant difference was found between the distribution of the GSTM1 (-) genotype in the two groups (chi2 = 0.831, P = 0.362). (2) When considered the GSTM1(+) genotype as reference, there was no overall statistically increased lung cancer risk for carriers with the GSTM1(-) genotype adjusted by age, gender and smoking status (OR = 0.840, 95% CI: 0.578-1.221, P = 0.362). (3) The frequency of the GSTM1(-) genotype for squamous cell carcinoma, adenocarcinoma, SCLC and other histological types was 65.8%, 48.5%, 47.8% and 52.2% respectively, compared with the control group, no statistically increased lung cancer risk was observed (P > 0.05).
No evidence is found between GSTMI genetic polymorphism and lung cancer risk among Han nationality population in Tianjin district.
谷胱甘肽S-转移酶M1（GSTM1）是一种重要的Ⅱ相代谢酶基因，参与人体多种致癌物的代谢。许多研究表明，GSTM1基因多态性与肺癌风险相关。本研究旨在探讨天津地区汉族人群中GSTM1基因多态性与肺癌风险的关系。
采用SYBR绿I实时荧光定量PCR法通过熔解曲线分析检测GSTM1基因多态性。本病例对照研究纳入265例经组织学确诊的肺癌患者和307名健康对照，研究GSTM1基因多态性与肺癌风险的关系。
（1）肺癌组和对照组中GSTMI（-）的频率分别为56.6%和57.0%，两组间GSTM1（-）基因型分布无显著差异（χ2 = 0.831，P = 0.362）。（2）以GSTM1（+）基因型为参照，经年龄、性别和吸烟状况校正后，GSTM1（-）基因型携带者的肺癌总体风险无统计学意义的增加（OR = 0.840，95%CI：0.578 - 1.221，P = 0.362）。（3）鳞状细胞癌、腺癌、小细胞肺癌和其他组织学类型中GSTM1（-）基因型的频率分别为65.8%、48.5%、47.8%和52.2%，与对照组相比，未观察到肺癌风险有统计学意义的增加（P > 0.05）。
在天津地区汉族人群中，未发现GSTMI基因多态性与肺癌风险之间存在关联。
