Dentler W L
Tissue Cell. 1977;9(2):209-22. doi: 10.1016/0040-8166(77)90017-9.
Cytochemical localization of ATPase activities in cilia and basal bodies of Tetrahymena pyriformis revealed a number of possible sites of ATPases. In basal bodies, reaction product was localized on the periphery of basal body microtubules, in the core of the B-microtubules, on the dense basal body core, and on the basal plate; some reaction product was associated with the postciliary and basal microtubules. In the cilium, reaction product was associated with the ciliary membrane, the basal granule, the periphery of the outer doublet microtubules, in the core of the B-microtubules, and on the arms and either the central microtubules or the radial spoke heads. Reaction product deposition required ATP and either Ca2+ or Mg2+ or ADP and Mg2+. When incubated in the presence of ATP and Na+, reaction product was only found at the base of the cilium in the region of the ciliary necklace. Implications of the various sites of activity are discussed with respect to possible mechanisms of ciliary motility.
梨形四膜虫纤毛和基体中ATP酶活性的细胞化学定位揭示了多个可能的ATP酶位点。在基体中，反应产物定位于基体微管的外周、B微管的核心、致密的基体核心以及基板上；一些反应产物与纤毛后微管和基体微管相关。在纤毛中，反应产物与纤毛膜、基部颗粒、外双联微管的外周、B微管的核心以及臂和中央微管或放射辐条头部相关。反应产物的沉积需要ATP以及Ca2+或Mg2+或ADP和Mg2+。当在ATP和Na+存在下孵育时，仅在纤毛项链区域的纤毛基部发现反应产物。针对纤毛运动的可能机制讨论了各种活性位点的意义。
