Department of Chemistry, Shantou University, Daxue Road, Shantou 515063, Guangdong, China.
Talanta. 2010 Aug 15;82(3):885-91. doi: 10.1016/j.talanta.2010.04.032. Epub 2010 Apr 24.
Ellagic acid (EA) reacted with Gemini zwitterionic surfactant, phosphodiesters quaternary ammonium salt (PQAS), and formed fine particles which produced strong enhancement in intensity of resonance light scattering (RLS). The effects of several factors on the RLS signal, such as pH, ionic strength, PQAS concentration and so on, were optimized. The relationship between enhanced RLS intensity and EA concentration was constructed. A novel and rapid method for the determination of EA was built. The linear range of this method was 0.016-4.0 microg mL(-1) and the detection limit was 13.9 ng mL(-1). Under the optimum conditions, the proposed method was applied to determine EA in body fluids with the results of quantitative recoveries between 98.4-101.4% in human serum samples and 99.1-102% in human urine samples. This method characterized by low limit detection is very sensitive and the cost is low, and constitutes a fast one-step procedure which requires only measuring the RLS intensities. The mechanism of the reaction was also studied. This investigation could contribute to the research on the delivery and release of bioactive molecules by Gemini surfactants.
鞣花酸（EA）与双子离子型表面活性剂、磷酸酯季铵盐（PQAS）反应，形成细小颗粒，使共振光散射（RLS）强度显著增强。优化了 pH 值、离子强度、PQAS 浓度等多种因素对 RLS 信号的影响。构建了增强 RLS 强度与 EA 浓度之间的关系。建立了一种用于测定 EA 的新的快速方法。该方法的线性范围为 0.016-4.0 μg mL（-1），检测限为 13.9 ng mL（-1）。在最佳条件下，该方法用于测定体液中的 EA，人血清样品的定量回收率在 98.4-101.4%之间，人尿液样品的回收率在 99.1-102%之间。该方法具有检测限低、灵敏度高、成本低的特点，是一种只需测量 RLS 强度的快速一步法。还研究了反应的机理。这项研究有助于研究双子表面活性剂对生物活性分子的传递和释放。
