Department of Chemistry, Shantou University, Shantou, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Oct 15;77(3):605-11. doi: 10.1016/j.saa.2010.06.023. Epub 2010 Jun 30.
The interaction between photosensitizer anticancer drug hematoporphyrin monomethyl ether (HMME) and ctDNA has been studied based on the decreased resonance light scattering (RLS) phenomenon. The RLS, UV-vis and fluorescence spectra characteristics of the HMME-ctDNA system were investigated. Besides, the phosphodiesters quaternary ammonium salt (PQAS), a kind of new gemini surfactant synthesized recently, was used to determine anticancer drug HMME based on the increasing RLS intensity. Under the optimum assay conditions, the enhanced RLS intensity was proportional to the concentration of HMME. The linear range was 0.8-8.4microgmL(-1), with correlation coefficient R(2)=0.9913. The detection limit was 0.014microgmL(-1). The human serum samples and urine samples were determined satisfactorily, which proved that this method was reliable and applicable in the determination of HMME in body fluid. The presented method was simple, sensitive and straightforward and could be a significant method in clinical analysis.
基于共振光散射（RLS）现象的降低，研究了光敏抗癌药物血卟啉单甲醚（HMME）与 ctDNA 之间的相互作用。研究了 HMME-ctDNA 体系的 RLS、紫外-可见和荧光光谱特征。此外，最近合成的一种新型双子表面活性剂磷酸二酯季铵盐（PQAS），基于 RLS 强度的增加，被用于测定抗癌药物 HMME。在最佳测定条件下，RLS 强度的增强与 HMME 的浓度成正比。线性范围为 0.8-8.4μgmL(-1)，相关系数 R(2)=0.9913。检测限为 0.014μgmL(-1)。对人血清样品和尿液样品进行了满意的测定，证明该方法在体液中 HMME 的测定中是可靠和适用的。所提出的方法简单、灵敏、直接，可作为临床分析的重要方法。
