Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunnkyo-ku, Tokyo 113-8510, Japan.
Talanta. 2010 Aug 15;82(3):892-8. doi: 10.1016/j.talanta.2010.04.048. Epub 2010 Apr 29.
A novel 2-dimensional spatiotemporal visualization system of expired gaseous ethanol after oral administration for real-time illustrated analysis of alcohol metabolism has been developed, which employed a low level light CCD camera to detect chemiluminescence (CL) generated by catalytic reactions of standard gaseous ethanol and expired gaseous ethanol after oral administration. First, the optimization of the substrates for visualization and the concentration of luminol solution for CL were investigated. The cotton mesh and 5.0 mmol L(-1) luminol solution were selected for further investigations and this system is useful for 0.1-20.0 mmol L(-1) of H(2)O(2) solution. Then, the effect of pH condition of Tris-HCl buffer solution was also evaluated with CL intensity and under the Tris-HCl buffer solution pH 10.1, a wide calibration range of standard gaseous ethanol (30-400 ppm) was obtained. Finally, expired air of 5 healthy volunteers after oral administration was measured at 15, 30, 45, 60, 75, 90, 105 and 120 min after oral administration, and this system showed a good sensitivity on expired gaseous ethanol for alcohol metabolism. The peaks of expired gaseous ethanol concentration appeared within 30 min after oral administration. During the 30 min after oral administration, the time variation profile based on mean values showed the absorption and distribution function, and the values onward showed the elimination function. The absorption and distribution of expired gaseous ethanol in 5 healthy volunteers following first-order absorption process were faster than the elimination process, which proves efficacious of this system for described alcohol metabolism in healthy volunteers. This system is expected to be used as a non-invasive method to detect VOCs as well as several other drugs in expired air for clinical purpose.
已开发出一种新型的二维时空可视化系统，用于实时分析口服后呼出气体中乙醇的代谢情况，该系统采用低强度光 CCD 摄像机检测标准气态乙醇和口服后呼出气体中乙醇的催化反应产生的化学发光（CL）。首先，优化了可视化的底物和鲁米诺溶液的浓度，以用于 CL 检测。选择了棉网和 5.0mmol/L 的鲁米诺溶液进行进一步研究，该系统可用于 0.1-20.0mmol/L 的 H2O2 溶液。然后，还评估了 Tris-HCl 缓冲溶液的 pH 值条件对 CL 强度的影响，在 Tris-HCl 缓冲溶液 pH 10.1 下，获得了标准气态乙醇（30-400ppm）的宽校准范围。最后，在口服后 15、30、45、60、75、90、105 和 120min 时测量了 5 名健康志愿者的呼出空气，该系统对呼出气体中乙醇的代谢具有良好的敏感性。呼出气体中乙醇浓度的峰值出现在口服后 30min 内。在口服后 30min 内，基于平均值的时间变化曲线显示了吸收和分布函数，而之后的值则显示了消除函数。5 名健康志愿者口服后，呼出气体中乙醇的吸收和分布过程快于消除过程，这证明了该系统在描述健康志愿者的酒精代谢方面是有效的。该系统有望作为一种非侵入性方法，用于检测呼出空气中的挥发性有机化合物（VOC）和其他几种药物，以用于临床目的。
