Vigh S, Arimura A, Köves K, Somogyvári-Vigh A, Sitton J, Fermin C D
U.S.-Japan Biomedical Research Laboratories, Tulane University Hebert Center, Belle Chasse, LA 70037.
Peptides. 1991 Mar-Apr;12(2):313-8. doi: 10.1016/0196-9781(91)90018-k.
A 38 residue neuropeptide was recently isolated from ovine hypothalamus in our laboratory, and named pituitary adenylate cyclase activating polypeptide (PACAP38) based on its biological activity. Rabbit antisera against synthetic PACAP27 were characterized by ELISA for immunohistochemical use. PACAP-immunoreactive neuronal elements having similar distributions were demonstrated in both human and spider monkey hypothalami. Many PACAP-immunoreactive cell bodies were present in the supraoptic and paraventricular nuclei. Immunopositive nerve fiber networks were stained throughout the hypothalamus, including in both external and internal zones of the tuber cinereum, close to the transition of the pituitary stalk (median eminence). These results suggest that PACAP plays multifunctional roles as a hypophysiotropic hormone, neurohypophysial hormone, neurotransmitter or neuromodulator in higher vertebrate species including man.
最近，我们实验室从羊下丘脑分离出一种38个氨基酸残基的神经肽，并根据其生物活性将其命名为垂体腺苷酸环化酶激活多肽（PACAP38）。通过酶联免疫吸附测定（ELISA）对针对合成PACAP27的兔抗血清进行了表征，以用于免疫组织化学。在人类和蜘蛛猴的下丘脑均证实了具有相似分布的PACAP免疫反应性神经元成分。视上核和室旁核中有许多PACAP免疫反应性细胞体。免疫阳性神经纤维网络在下丘脑各处均被染色，包括灰结节的外部和内部区域，靠近垂体柄（正中隆起）的过渡处。这些结果表明，PACAP在包括人类在内的高等脊椎动物物种中作为促垂体激素、神经垂体激素、神经递质或神经调节剂发挥多种功能。
