斑马鱼胚胎中 PAC1-R 和 PACAP 基因的表达分析。
Expression analysis of PAC1-R and PACAP genes in zebrafish embryos.
机构信息
INSERM U982, University of Rouen, Mont-Saint-Aignan, France.
出版信息
J Mol Neurosci. 2011 Jan;43(1):94-100. doi: 10.1007/s12031-010-9397-y. Epub 2010 Jun 5.
Abstract
This study describes the expression of the pituitary adenylate cyclase-activating polypeptide (PACAP1 and PACAP2) and PAC1 receptor genes (PAC1a-R and PAC1b-R) in the brain of zebrafish (Danio rerio) during development. In situ hybridization of the 24- and 48-hpf embryos revealed that PACAP genes were expressed in the telencephalon, the diencephalon, the rhombencephalon, and the neurons in the dorsal part of the spinal cord. PACAP2 mRNA appears to be the most abundant form during brain development. The two PAC1-R subtypes showed a similar expression pattern: mRNAs were detected in the forebrain, the thalamus, and the rhombencephalon. However, in the tectum, only PAC1b-R gene was detected. These results suggest that, in fish, PACAP may play a role in brain development.
摘要
本研究描述了在斑马鱼（Danio rerio）发育过程中脑垂体腺苷酸环化酶激活肽（PACAP1 和 PACAP2）和 PAC1 受体基因（PAC1a-R 和 PAC1b-R）的表达。对 24 和 48 小时胚胎的原位杂交显示，PACAP 基因在端脑、间脑、后脑和脊髓背部分化神经元中表达。在脑发育过程中，PACAP2 mRNA 似乎是最丰富的形式。两种 PAC1-R 亚型表现出相似的表达模式：在大脑前脑、丘脑和后脑中检测到 mRNAs。然而，在顶盖中仅检测到 PAC1b-R 基因。这些结果表明，在鱼类中，PACAP 可能在脑发育中发挥作用。
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