Section for Parasitology, Health and Development, Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Dyrlaegevej 100 Frederiksberg, 1870, Copenhagen, Denmark.
Parasitol Res. 2010 Nov;107(6):1323-7. doi: 10.1007/s00436-010-2004-x. Epub 2010 Aug 3.
This experiment has been conducted to evaluate the viability of Angiostrongylus vasorum L1 under different conditions of temperature and humidity. In order to assess the viability, fox faecal pellets containing first stage larvae (L1) were exposed to relative humidity (RH) 95% and 75%, and to different temperatures (5°C, 18°C) and at fluctuating conditions ranging from -5°C to +5°C. Moreover, larval viability under outdoor conditions in April was also observed. Survival of the larvae was strongly influenced by temperature; however, humidity did not show any significant influence on viability. In controlled condition, 100% of the larvae were found motile and active; whereas, around 14% and 19% of the larvae kept at 5°C remained viable at RH 95% and RH 75%, respectively after 78 h. All of the larvae kept at 18°C died after 66 h. In outdoor condition, larval survivability reduced to 18% after 66 h, and in fluctuation temperature viable larvae were observed in both control and experimental conditions (15%) after 78 h.
本实验旨在评估血管圆线虫 L1 在不同温度和湿度条件下的生存能力。为了评估生存能力，含有第一期幼虫（L1）的狐狸粪便被暴露在相对湿度（RH）为 95%和 75%，以及不同的温度（5°C、18°C）和从-5°C 到+5°C 的波动条件下。此外，还观察了 4 月户外条件下幼虫的生存能力。幼虫的存活受温度影响很大；然而，湿度对其活力没有显著影响。在控制条件下，100%的幼虫表现出活跃和活跃；然而，在 5°C 下，RH 为 95%和 RH 为 75%的条件下，分别有 14%和 19%的幼虫在 78 小时后仍具有活力。所有在 18°C 下的幼虫在 66 小时后死亡。在户外条件下，66 小时后幼虫的存活率降低到 18%，在波动温度下，78 小时后在对照和实验条件下均观察到有活力的幼虫（15%）。
