Department of Anatomy and Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea.
Mol Cells. 2010 Aug;30(2):161-5. doi: 10.1007/s10059-010-0102-3. Epub 2010 Jul 23.
Regulator of G-protein signaling (RGS) proteins play an important role in G-protein coupled receptor (GPCR) signaling and the activity of some GPCRs is modulated via RGS protein levels during stress response. The aim of this study was to investigate changes in RGS protein mRNA expressions in the mouse brain after 2h restraint stress. The mRNA level of 19 RGS proteins was analyzed using real-time PCR in six brain regions, which included the prefrontal cortex, amygdala, hippocampus, hypothalamus, striatum, and pituitary gland, from control and stressed mouse. We found that the level of mRNA of each RGS varied according to brain region and that two to eight RGS proteins exhibited changes in mRNA levels in each brain region by restraint stress. It was also revealed that RGS4 protein amount was consistent with mRNA level, indicating RGS4 protein may have regulatory roles in the acute stress response.
G 蛋白信号调节蛋白（RGS 蛋白）在 G 蛋白偶联受体（GPCR）信号转导中发挥重要作用，一些 GPCR 的活性可通过应激反应过程中 RGS 蛋白水平进行调节。本研究旨在探讨 2 小时束缚应激后小鼠大脑中 RGS 蛋白 mRNA 表达的变化。采用实时 PCR 法检测了 6 个脑区（前额叶皮层、杏仁核、海马体、下丘脑、纹状体和垂体）中 19 种 RGS 蛋白的 mRNA 水平，包括对照和应激小鼠。结果发现，每种 RGS 的 mRNA 水平均因脑区而异，并且有 2 至 8 种 RGS 蛋白在每个脑区的 mRNA 水平因束缚应激而发生变化。还揭示了 RGS4 蛋白的含量与 mRNA 水平一致，表明 RGS4 蛋白可能在急性应激反应中具有调节作用。
