Altelaar A F Maarten, Piersma Sander R
Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, Utrecht, The Netherlands.
Methods Mol Biol. 2010;656:197-208. doi: 10.1007/978-1-60761-746-4_11.
Imaging mass spectrometry (IMS) allows the direct investigation of both the identity and the spatial distribution of the entire molecular content directly in tissue sections, single cells, and many other biological surfaces. We describe here the steps required to retrieve the molecular information from tissue sections using matrix-enhanced (ME) and metal-assisted (MetA) secondary ion mass spectrometry (SIMS). Surface metallization by plasma coating enhances desorption/ionization of membrane components such as lipids and sterols in imaging time-of-flight (ToF) SIMS of tissues and cells. High-resolution images of cholesterol and other membrane components can be obtained for single neuroblastoma cells and reveal subcellular details. Alternatively, in ME-SIMS, 2,5-dihydroxybenzoic acid electrosprayed on neuroblastoma cells allows intact molecular ion imaging of phosphatidylcholine (PC) and sphingomyelin (SM) at the cellular level.
成像质谱（IMS）能够直接在组织切片、单细胞以及许多其他生物表面上，对整个分子成分的身份和空间分布进行直接研究。我们在此描述了使用基质增强（ME）和金属辅助（MetA）二次离子质谱（SIMS）从组织切片中获取分子信息所需的步骤。通过等离子体涂层进行表面金属化，可增强组织和细胞成像飞行时间（ToF）SIMS中脂质和固醇等膜成分的解吸/电离。对于单个神经母细胞瘤细胞，可以获得胆固醇和其他膜成分的高分辨率图像，并揭示亚细胞细节。或者，在ME-SIMS中，电喷雾在神经母细胞瘤细胞上的2,5-二羟基苯甲酸能够在细胞水平上对磷脂酰胆碱（PC）和鞘磷脂（SM）进行完整分子离子成像。
