Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa,
Cytotechnology. 2010 Jul;62(3):265-77. doi: 10.1007/s10616-010-9293-x. Epub 2010 Aug 3.
The abalone, Haliotis midae, is the most valuable commodity in South African aquaculture. The increasing demand for marine shellfish has stimulated research on the biology and physiology of target species in order to improve knowledge on growth, nutritional requirements and pathogen identification. The slow growth rate and long generation time of abalone restrict efficient design of in vivo experiments. Therefore, in vitro systems present an attractive alternative for short term experimentation. The use of marine invertebrate cell cultures as a standardised and controlled system to study growth, endocrinology and disease contributes to the understanding of the biology of economically important molluscs. This paper investigates the suitability of two different H. midae tissues, larval and haemocyte, for establishing primary cell cultures. Cell cultures are assessed in terms of culture initiation, cell yield, longevity and susceptibility to contamination. Haliotis midae haemocytes are shown to be a more feasible tissue for primary cell culture as it could be maintained without contamination more readily than larval cell cultures. The usefulness of short term primary haemocyte cultures is demonstrated here with a growth factor trial. Haemocyte cultures can furthermore be used to relate phenotypic changes at the cellular level to changes in gene expression at the molecular level.
鲍鱼是南非水产养殖中最有价值的商品。对海洋贝类的需求不断增加，这刺激了对目标物种生物学和生理学的研究，以提高对生长、营养需求和病原体鉴定的认识。鲍鱼的生长速度慢和世代时间长限制了体内实验的有效设计。因此，体外系统为短期实验提供了一个有吸引力的选择。使用海洋无脊椎动物细胞培养作为研究生长、内分泌和疾病的标准化和受控系统，有助于理解经济上重要的软体动物的生物学。本文研究了两种不同的 H. midae 组织（幼虫和血细胞）建立原代细胞培养的适宜性。从培养起始、细胞产量、寿命和易污染性等方面评估细胞培养。结果表明，Haliotis midae 血细胞是更适合原代细胞培养的组织，因为它比幼虫细胞培养更容易在没有污染的情况下维持。本文通过生长因子试验证明了短期原代血细胞培养的有用性。血细胞培养还可用于将细胞水平的表型变化与分子水平的基因表达变化联系起来。
