JenLab GmbH, Schillerstraße 1, 07745 Jena and Campus A1.2, 66123 Saarbrücken, Germany.
J Biophotonics. 2010 Dec;3(12):759-73. doi: 10.1002/jbio.201000074.
The first-ever application of high-frequency ultrasound combined with multiphoton tomography (MPT) and dermoscopy in a clinical trial is reported. 47 patients with different dermatoses such as benign and malign skin cancers, connective tissue diseases, inflammatory skin diseases, and autoimmune bullous skin diseases have been investigated with (i) state-of-the-art and highly sophisticated ultrasound systems for dermatology, (ii) the femtosecond laser multiphoton tomograph and (iii) dermoscopes. Dermoscopy provides two-dimensional color images of the skin surface with a magnification up to 70 x. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide-field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 μm (axial) and 32 μm (lateral). Multiphoton tomography provides 0.36 x 0.36 x 0.001 mm³ horizontal optical sections of a particular region of interest with submicron resolution down to 200 μm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments.
首次将高频超声与多光子断层扫描（MPT）和皮肤镜检查应用于临床试验的报告。对 47 例不同皮肤病患者（如良性和恶性皮肤癌、结缔组织疾病、炎症性皮肤病和自身免疫性大疱性皮肤病）进行了研究，使用了（i）最先进和高度复杂的皮肤科超声系统，（ii）飞秒激光多光子断层扫描仪和（iii）皮肤镜。皮肤镜提供皮肤表面的二维彩色图像，放大倍数高达 70x。根据从 7.5MHz 到 100MHz 的超声频率，信号深度从大约 1mm 到 80mm 不等。垂直超声宽场图像可快速提供病变的深度和体积信息。100MHz 超声允许以低至 16μm（轴向）和 32μm（侧向）的分辨率进行成像。多光子断层扫描提供特定感兴趣区域的 0.36x0.36x0.001mm³水平光学切片，具有亚微米分辨率，可达 200μm 组织深度。线粒体辅酶、角蛋白、黑色素和弹性蛋白的自发荧光以及胶原结构网络都可以成像。超声和 MPT 的结合为皮肤病的诊断开辟了新的协同可能性，特别关注皮肤癌的早期检测以及治疗效果的评估。
