Biochemistry Department, University of Otago, Dunedin 9054, New Zealand.
Biochem J. 2010 Oct 1;431(1):23-9. doi: 10.1042/BJ20100957.
RNF4 [RING (really interesting new gene) finger protein 4] family ubiquitin ligases are RING E3 ligases that regulate the homoeostasis of SUMOylated proteins by promoting their ubiquitylation. In the present paper we report that the RING domain of RNF4 forms a stable dimer, and that dimerization is required for ubiquitin transfer. Our results suggest that the stability of the E2~ubiquitin thioester bond is regulated by RING domain dimerization.
RNF4 [RING (really interesting new gene) 指蛋白 4] 家族泛素连接酶是 RING E3 泛素连接酶,通过促进 SUMO 化蛋白的泛素化来调节其同源性。在本文中,我们报告 RNF4 的 RING 结构域形成稳定的二聚体,并且二聚化是泛素转移所必需的。我们的结果表明,E2~泛素硫酯键的稳定性受 RING 结构域二聚化的调节。