开曼群岛埃及伊蚊的拟除虫菊酯抗性。
Pyrethroid resistance in Aedes aegypti from Grand Cayman.
机构信息
The Mosquito Research and Control Unit, Grand Cayman, Cayman Islands, British West Indies.
出版信息
Am J Trop Med Hyg. 2010 Aug;83(2):277-84. doi: 10.4269/ajtmh.2010.09-0623.
Abstract
The Grand Cayman population of Aedes aegypti is highly resistant to DDT and pyrethroid insecticides. Glutathione transferase, cytochrome P450, and esterase levels were increased in the Grand Cayman population relative to a susceptible laboratory strain, but synergist studies did not implicate elevated insecticide detoxification as a major cause of resistance. The role of target site resistance was therefore investigated. Two substitutions in the voltage-gated sodium channel were identified, V1016I in domain II, segment 6 (IIS6) (allele frequency = 0.79) and F1534C in IIIS6 (allele frequency = 0.68). The role of the F1534C mutation in conferring resistance to insecticides has not been previously established and so a tetraplex polymerase chain reaction assay was designed and used to genotype mosquitoes that had been exposed to insecticides. The F1534C mutation was strongly correlated with resistance to DDT and permethrin.
摘要
开曼群岛的埃及伊蚊种群对滴滴涕和拟除虫菊酯杀虫剂具有高度抗性。与敏感的实验室品系相比，开曼群岛种群的谷胱甘肽转移酶、细胞色素 P450 和酯酶水平升高，但增效剂研究并未表明升高的杀虫剂解毒作用是产生抗药性的主要原因。因此，研究了靶标抗性的作用。在电压门控钠离子通道中鉴定出两个取代，II 域 6 节中的 V1016I（IIS6）（等位基因频率=0.79）和 IIIS6 中的 F1534C（等位基因频率=0.68）。F1534C 突变赋予对杀虫剂抗性的作用以前尚未确定，因此设计并使用四重聚合酶链反应检测来对已经接触过杀虫剂的蚊子进行基因分型。F1534C 突变与滴滴涕和氯菊酯的抗性密切相关。
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