Soni Monika, Bhattacharya Chandrakanta, Sharma Jitendra, Dutta Prafulla
Division of Entomology and Filariasis, Regional Medical Research Centre (ICMR), Dibrugarh, Assam, India.
District Surveillance Unit, Office of the Joint Director of Health Services, Lakhimpur, Assam, India.
Indian J Med Microbiol. 2018 Jul-Sep;36(3):435-438. doi: 10.4103/ijmm.IJMM_18_120.
Nowadays, dengue infection creates a major problem across the country. The vector species carrying dengue infection has progressively started to developed resistance against most of the currently used insecticides. Hence, a study was carried out in dengue-endemic areas of Assam and Arunachal Pradesh to find the current situation of insecticide susceptibility status of dengue vectors. Based on the previous history of dengue incidence, Aedes mosquitoes were collected from Dibrugarh, Kamrup, Sivasagar, Tezpur and Tinsukia districts in Assam and Pasighat district in Arunachal Pradesh to test the insecticide resistance status through bioassay and molecular methods. The WHO standard bioassay test kits were used to detect insecticide susceptibility status among dengue vectors. In molecular study, allele-specific polymerase chain reaction (PCR) method was done for the detection of mutations in paratype voltage-gated sodium channel gene of Aedes aegypti and Aedes albopictus mosquitoes. In bioassay method, 100% A. aegypti mosquitoes were found to be resistant towards dichlorodiphenyltrichloroethane (DDT), 8% towards pyrethroid and 4% towards malathion. Similarly, 92% A. albopictus mosquitoes have shown resistance competency towards DDT, 12% towards pyrethroid and 8% towards malathion. In allele-specific PCR methods, V1016G heterozygous mutations were detected from the field collected A. aegypti and A. albopictus mosquitoes of Tinsukia, Dibrugarh and Sivasagar district. Similarly, F1534C heterozygous mutations were observed from A. aegypti mosquitoes of Tezpur, Tinsukia and Sivasagar district and A. albopictus mosquitoes of Tinsukia, Dibrugarh and Sivasagar district. From the study, it was concluded that the Aedes mosquitoes have progressively started to developed resistance towards commonly used insecticides.
如今，登革热感染在全国造成了一个重大问题。携带登革热感染的病媒物种已逐渐开始对目前使用的大多数杀虫剂产生抗性。因此，在阿萨姆邦和阿鲁纳恰尔邦的登革热流行地区开展了一项研究，以了解登革热媒介对杀虫剂的易感性现状。根据登革热发病的既往史，从阿萨姆邦的迪布鲁格尔、卡姆鲁普、锡瓦萨加尔、提斯普尔和廷苏基亚地区以及阿鲁纳恰尔邦的帕西格哈特地区收集伊蚊，通过生物测定和分子方法检测其抗药性状况。使用世界卫生组织标准生物测定测试试剂盒来检测登革热媒介中的杀虫剂易感性状况。在分子研究中，采用等位基因特异性聚合酶链反应（PCR）方法检测埃及伊蚊和白纹伊蚊副型电压门控钠通道基因的突变。在生物测定方法中，发现100%的埃及伊蚊对滴滴涕（DDT）具有抗性，8%对拟除虫菊酯具有抗性，4%对马拉硫磷具有抗性。同样，92%的白纹伊蚊对DDT表现出抗药性，12%对拟除虫菊酯具有抗性，8%对马拉硫磷具有抗性。在等位基因特异性PCR方法中，从廷苏基亚、迪布鲁格尔和锡瓦萨加尔地区野外采集的埃及伊蚊和白纹伊蚊中检测到V1016G杂合突变。同样，在提斯普尔、廷苏基亚和锡瓦萨加尔地区的埃及伊蚊以及廷苏基亚、迪布鲁格尔和锡瓦萨加尔地区的白纹伊蚊中观察到F1534C杂合突变。从该研究得出结论，伊蚊已逐渐开始对常用杀虫剂产生抗性。
