State Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou, China.
J Proteome Res. 2010 Oct 1;9(10):5053-64. doi: 10.1021/pr100356t.
Diapause is a period of developmental arrest that allows a species to adapt to unfavorable conditions. Many insect species reduce metabolic activity and then enter diapause at a certain stage in their life cycles. The cotton bollworm, Helicoverpa armigera, will be destined for pupal diapause when larvae are reared under short daylengths and low temperature. The brain is an important organ for diapause decision, and some signaling molecules from the brain of diapause-destined individuals are released into the hemolymph to regulate diapause. In this study, we performed 2-D gel-based comparative proteomic and phosphoproteomic analyses to search for differentially expressed proteins between nondiapause- and diapause-destined pupal brains. A total of 79 proteins and 23 phosphoproteins showed significant differences between these two groups, and 41 proteins and 10 phosphoproteins were identified by MALDI-TOF/TOF MS. Further, gene expression patterns in diapause- and nondiapause-destined pupal brains were confirmed by RT-PCR or Western blot analysis. These differentially expressed proteins act in the metabolic change, stress response, and signal transduction pathways at early pupal stage for diapause initiation. Thus, these identified proteins may depress metabolism in diapause-destined pupae to lead the insect to enter developmental arrest.
滞育是一种发育停滞的时期，使物种能够适应不利的条件。许多昆虫物种会降低代谢活性，然后在其生命周期的某个阶段进入滞育。当幼虫在短日照和低温下饲养时，棉铃虫将注定要进入蛹滞育。大脑是滞育决策的重要器官，一些来自注定要滞育的个体大脑的信号分子被释放到血液中，以调节滞育。在这项研究中，我们进行了基于 2-D 凝胶的比较蛋白质组学和磷酸蛋白质组学分析，以寻找非滞育和滞育预定蛹脑中差异表达的蛋白质。两组之间共有 79 种蛋白质和 23 种磷酸蛋白质存在显著差异，通过 MALDI-TOF/TOF MS 鉴定了 41 种蛋白质和 10 种磷酸蛋白质。此外，通过 RT-PCR 或 Western blot 分析证实了滞育和非滞育预定蛹脑中的基因表达模式。这些差异表达的蛋白质在早期蛹期的代谢变化、应激反应和信号转导途径中起作用，以启动滞育。因此，这些鉴定出的蛋白质可能会降低滞育预定蛹的代谢，使昆虫进入发育停滞。
