Dong Yong-Cheng, Chen Zhen-Zhong, Clarke Anthony R, Niu Chang-Ying
Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection, Anhui Agricultural University, Hefei, China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Front Physiol. 2019 Oct 30;10:1288. doi: 10.3389/fphys.2019.01288. eCollection 2019.
Correct timing of diapause entry and exit is critical for a species' survival. While many aspects of insect diapause are well-studied, the mechanisms underlying diapause termination remain largely unknown. The Chinese citrus fly, , is a univoltine insect with an obligatory pupal diapause. The application of 20-hydroxyecdysone (20E) is known to terminate diapause in , and we used this approach, along with isobaric tags for relative and absolute quantitation technology, to determine the proteins associated with diapause termination in this fly. Among 2,258 identified proteins, 1,169 proteins significantly differed at 1, 2, and 5 days post-injection of 20E, compared with the solvent-injected control group. Functional annotation revealed that the majority of differentially expressed proteins were enriched in the core energy metabolism of amino acids, proteins, lipids, and carbohydrates as well as in signal transduction pathways including PPAR signaling, Calcium signaling, Glucagon signaling, VEGF signaling, Ras signaling, cGMP-PKG signaling, and cAMP signaling. A combined transcriptomic and proteomic analysis suggested the involvement of energy metabolism in the response of diapause transition. RNA interference experiments disclosed that a 20E injection triggers diapause termination probably through non-genomic actions, rather than nuclear receptor mediated genomic actions. Our results provide extensive proteomic resources for insect diapause transition and offer a potential for pest control by incapacitating the regulation of diapause termination either by breaking diapause prematurely or by delaying diapause termination to render diapausing individuals at a high risk of mortality.
滞育进入和退出的正确时机对物种的生存至关重要。虽然昆虫滞育的许多方面都得到了充分研究，但滞育终止的潜在机制在很大程度上仍然未知。柑橘大实蝇是一种一年发生一代、蛹期滞育的昆虫。已知应用20-羟基蜕皮酮（20E）可终止柑橘大实蝇的滞育，我们采用这种方法，并结合相对和绝对定量的等压标记技术，来确定与该果蝇滞育终止相关的蛋白质。在鉴定出的2258种蛋白质中，与注射溶剂的对照组相比，在注射20E后1天、2天和5天，有1169种蛋白质存在显著差异。功能注释显示，大多数差异表达的蛋白质在氨基酸、蛋白质、脂质和碳水化合物的核心能量代谢以及包括PPAR信号传导、钙信号传导、胰高血糖素信号传导、VEGF信号传导、Ras信号传导、cGMP-PKG信号传导和cAMP信号传导在内的信号转导途径中富集。转录组学和蛋白质组学的联合分析表明能量代谢参与了滞育转变反应。RNA干扰实验表明，注射20E可能通过非基因组作用而非核受体介导的基因组作用触发滞育终止。我们的结果为昆虫滞育转变提供了丰富的蛋白质组学资源，并为害虫防治提供了一种可能性，即通过过早打破滞育或延迟滞育终止以使滞育个体面临高死亡风险来破坏滞育终止的调节。
