Briaud B, Koch B, Lutz-Bucher B, Mialhe C
Neuroendocrinology. 1978;25(1):47-63. doi: 10.1159/000122728.
Using incubated glands, we showed that cerebral cortex and liver extracts (CCE and LE) stimulated ACTH release from neurointermediate lobe (NIL) of hypophysis as well as hypothalmus extract (HE) did. Moreover, the HE-induced ACTH release was much smaller for the NIL (1.9 X basal level) than for the anterior lobe (AL; 13.7 x basal level). Thus, under these conditions, HE seemed to have no specific effect on NIL ACTH release. Using superfused glands, we showed: (a) that both spontaneous and HE-induced ACTH release decreased during superfusion; (b) that using this system, a specific stimulatory effect on HE on NIL was observed. In contrast to HE, CCE and LE had only a small effect on NIL ACTH release (always less than 20% of that caused by HE) which could be considered as a nonspecific response; (c) that trypsin suppressed the stimulating effect on HE as well on NIL as on AL; and (d) that arginine antidiuretic hormone (ADH) was not responsible for the stimulating effect of HE on NIL ACTH release, because synthetic ADH had no effect and HE containing ADH (from normal rats) or HE containing no ADH (from Brattleboro rats or from immunoneutralization of ADH in normal HE) had the same effect. From these results, we can conclude that HE contain a peptidic factor different from ADH which is able to stimulate in vitro release of ACTH from the NIL.
利用孵育的腺体，我们发现大脑皮层和肝脏提取物（CCE和LE）与下丘脑提取物（HE）一样，都能刺激垂体神经中间叶（NIL）释放促肾上腺皮质激素（ACTH）。此外，HE诱导的NIL的ACTH释放量（为基础水平的1.9倍）远低于前叶（AL；为基础水平的13.7倍）。因此，在这些条件下，HE似乎对NIL的ACTH释放没有特异性作用。利用灌流腺体，我们发现：（a）在灌流过程中，自发的和HE诱导的ACTH释放均减少；（b）利用该系统，观察到HE对NIL有特异性刺激作用。与HE不同，CCE和LE对NIL的ACTH释放仅有很小的影响（始终小于HE引起的释放量的20%），可将其视为非特异性反应；（c）胰蛋白酶抑制了HE对NIL以及对AL的刺激作用；（d）精氨酸抗利尿激素（ADH）与HE对NIL的ACTH释放的刺激作用无关，因为合成ADH无作用，且含有ADH的HE（来自正常大鼠）或不含ADH的HE（来自布拉特洛维大鼠或正常HE中ADH经免疫中和处理后的产物）具有相同的作用。从这些结果中，我们可以得出结论，HE含有一种不同于ADH的肽类因子，该因子能够在体外刺激NIL释放ACTH。
