International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Dhaka, Bangladesh.
Int J Food Microbiol. 2010 Sep 1;142(3):375-8. doi: 10.1016/j.ijfoodmicro.2010.07.019. Epub 2010 Jul 16.
Cronobacter spp. formerly known as Enterobacter sakazakii is an occasional contaminant of powdered infant formula (PIF). This pathogen has been associated with out-breaks of a rare form of infant meningitis, necrotizing enterocolitis (NEC), bacteremia and neonate deaths. The organism is ranked by the International Commission for Microbiological Specifications for Foods (ICMSF) as a 'Severe hazard for restricted populations, life threatening or substantial chronic sequelae or long duration'. Present study aimed to isolate Cronobacter spp. from PIF and clinical samples, such as blood, stool and CSF collected from 93 neonates and child patients, age ranged from 0 to 24months. We did not detect Cronobacter spp. in any of these samples. Later 32 PIF samples collected from retail markets in Bangladesh were tested for the presence of Cronobacter spp. Of these only one was found to be contaminated with Cronobacter sp. This is the first case of Cronobacter contaminated PIF found in Bangladesh to be reported. The organism was successfully identified based on its typical culture characteristics, producing blue-green colonies on chromogenic DFI agar and also by a standardized conventional PCR assay targeting the alpha glucosidase and 16S rRNA gene sequence of Cronobacter sp. The 16S rRNA gene was partially sequenced to provide for the phylogenetic analysis of this isolate (DA01) and found to cluster with some other Cronobacter isolates in the phylogram.
阪崎克罗诺杆菌（Cronobacter spp.）以前被称为阴沟肠杆菌（Enterobacter sakazakii），是配方奶粉（PIF）中的一种偶发污染物。这种病原体与婴儿脑膜炎、坏死性小肠结肠炎（NEC）、菌血症和新生儿死亡的爆发有关。该病原体被国际食品微生物规格委员会（ICMSF）列为“对特定人群具有严重危害、威胁生命或造成严重慢性后遗症或持续时间长”的病原体。本研究旨在从 93 名 0 至 24 个月大的新生儿和儿童患者的血液、粪便和脑脊液等临床样本以及 PIF 中分离阪崎克罗诺杆菌。我们在这些样本中均未检测到阪崎克罗诺杆菌。随后对从孟加拉国零售市场采集的 32 份 PIF 样本进行了阪崎克罗诺杆菌的检测。其中只有一份样本被发现受到了阪崎克罗诺杆菌的污染。这是孟加拉国首次报告发现受污染的 PIF 中存在阪崎克罗诺杆菌。该病原体是根据其典型的培养特征成功鉴定的，在显色 DFI 琼脂上产生蓝绿色菌落，也可以通过针对阪崎克罗诺杆菌的α葡萄糖苷酶和 16S rRNA 基因序列的标准化常规 PCR 检测来鉴定。对 16S rRNA 基因进行了部分测序，以便对该分离株（DA01）进行系统发育分析，并在系统发育树中与其他一些阪崎克罗诺杆菌分离株聚类。
