Department of Environmental and Preventive Medicine, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Osaka 565-0871, Japan.
Mutat Res. 2010 Aug 30;701(2):132-6. doi: 10.1016/j.mrgentox.2010.05.013. Epub 2010 Jun 1.
The aim of this study was to investigate the effects of alcohol drinking and ADH1B and ALDH2 polymorphisms on basal DNA damage (measured by the alkaline comet assay) of mononuclear cells in 122 healthy Japanese workers. Our results showed that drinking frequency had a significant impact on the tail moment (TM) value, with the highest TM value observed in habitual drinkers. The presence of the ADH1B2 or ALDH22 allele was associated with increased DNA damage in older habitual drinkers. Furthermore, habitual drinkers with a combined genotype of ADH1B*2/2 and ALDH21/*2 demonstrated a significantly higher TM value than other groups. Moreover, the combination of drinking and smoking has a combined effect on DNA damage. Multiple regression analysis revealed that drinking frequency, smoking status, and ALDH2 polymorphisms significantly influence basal TM value, suggesting that these are important variables affecting individual basal DNA damage.
本研究旨在探讨饮酒以及 ADH1B 和 ALDH2 多态性对 122 名健康日本工人单核细胞基础 DNA 损伤（通过碱性彗星试验测量）的影响。我们的结果表明，饮酒频率对尾矩（TM）值有显著影响，习惯性饮酒者的 TM 值最高。ADH1B2 或 ALDH22 等位基因的存在与年龄较大的习惯性饮酒者的 DNA 损伤增加有关。此外，ADH1B*2/2 和 ALDH21/*2 基因型组合的习惯性饮酒者的 TM 值明显高于其他组。此外，饮酒和吸烟的组合对 DNA 损伤有联合作用。多元回归分析表明，饮酒频率、吸烟状况和 ALDH2 多态性显著影响基础 TM 值，提示这些是影响个体基础 DNA 损伤的重要变量。
