Department of Chemistry, Nankai University, Tianjin 300071, PR China.
Talanta. 2010 Jun 30;82(1):404-8. doi: 10.1016/j.talanta.2010.03.016. Epub 2010 Mar 19.
A simple one-step in situ "click" modification strategy was developed for the preparation of hydrophobic organic monolithic columns for the first time. The column morphology and surface chemistry of the fabricated monolithic columns were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, respectively. The chromatographic performances of the C8/C18 "click" monoliths were evaluated through the separation of a mixture of five proteins such as ribonuclease A, soybean trypsin inhibitor, cytochrome c, bovine haemoglobin and bovine serum albumin. Compared with the blank column, the higher hydrophobicity stationary phases obtained from the "clicked" modification have longer retention times and higher resolution for the five proteins. The separation of five proteins mixture on click C18 monolith with gradient elution at different flow rates was also investigated, the baseline separation of five proteins could be achieved at three different flow rates.
首次开发了一种简单的一步原位“点击”修饰策略，用于制备疏水性有机整体柱。通过扫描电子显微镜、傅里叶变换红外光谱和 X 射线光电子能谱分别对制备的整体柱的柱形态和表面化学性质进行了表征。通过分离核糖核酸酶 A、大豆胰蛋白酶抑制剂、细胞色素 c、牛血红蛋白和牛血清白蛋白等五种蛋白质的混合物，评估了 C8/C18“点击”整体柱的色谱性能。与空白柱相比，“点击”修饰得到的高疏水性固定相对五种蛋白质具有更长的保留时间和更高的分辨率。还研究了在不同流速下进行梯度洗脱时点击 C18 整体柱上五种蛋白质混合物的分离情况，在三种不同的流速下可以实现五种蛋白质的基线分离。
