Department of Chemistry, Nankai University, Tianjin 300071, China.
Anal Bioanal Chem. 2011 Apr;399(10):3407-13. doi: 10.1007/s00216-010-4390-4. Epub 2010 Nov 16.
Two types of macroporous organic polymer monoliths based on glycidyl methacrylate (GMA), 4-vinylbenzyl chloride (VBC) and divinylbenzene (DVB) were prepared inside stainless-steel tubes. Azide functionalities were firstly introduced on the surfaces of poly(GMA-co-DVB) and poly(VBC-co-DVB) monoliths to provide reactive sites for click chemistry. With the application of copper(I)-catalyzed (3 + 2) azide-alkyne cycloaddition, an in-column click-modification approach for covalent attachment of long alkyl chains onto polymer monoliths was developed. The column morphology and surface chemistry of the fabricated monolithic columns were characterized by the scanning electron microscopy, mercury intrusion porosimeter, Fourier transform infrared spectroscopy, and elemental analyses, respectively. The chromatographic performances of the "clicked" stationary phases were demonstrated with the high separation efficiency for a variety of proteins within 4 min.
两种基于甲基丙烯缩水甘油酯（GMA）、4-乙烯基苄氯（VBC）和二乙烯基苯（DVB）的大孔有机聚合物整体柱是在不锈钢管内制备的。叠氮基官能团首先被引入聚（GMA-co-DVB）和聚（VBC-co-DVB）整体柱的表面，为点击化学提供反应位点。应用铜（I）催化的（3 + 2）叠氮-炔环加成反应，开发了一种在柱内点击修饰方法，将长链烷基共价键合到聚合物整体柱上。通过扫描电子显微镜、压汞孔隙率计、傅里叶变换红外光谱和元素分析分别对制备的整体柱的柱形态和表面化学性质进行了表征。通过在 4 分钟内对多种蛋白质进行高效分离，证明了“点击”固定相的色谱性能。
