Lee B M, Yin B Y, Herbert R, Hemminki K, Perera F P, Santella R M
College of Pharmacy, Sung Kyun Kwan University, Su-Won, South Korea.
Scand J Work Environ Health. 1991 Jun;17(3):190-4. doi: 10.5271/sjweh.1711.
Roofers and iron foundry workers with high exposure to polycyclic aromatic hydrocarbons (PAH) were monitored for levels of covalent PAH serum albumin adducts, quantitated in enzymatically digested samples by a sensitive competitive enzyme-linked immunosorbent assay. Albumin adducts were higher in the foundry workers (5.22 fmol/micrograms, 0.314 mmol/mol) than in the reference group (4.07 fmol/micrograms, 0.245 mmol/mol), but only of borderline significance probably due to the small sample size. In a subset of foundry workers, a significant difference in adduct levels was observed for samples collected immediately after vacation and after six weeks of workplace exposure. The roofers also showed higher levels of adducts (5.19 fmol/micrograms, 0.312 mmol/mol) than their reference group (3.28 fmol/micrograms, 0.197 mmol/mol). These results demonstrate the feasibility of PAH protein adduct measurement as a marker of human exposure to this class of chemicals.
对多环芳烃（PAH）暴露量高的屋顶工和铸铁厂工人进行了监测，检测其血清白蛋白共价PAH加合物水平，通过灵敏的竞争性酶联免疫吸附测定法对酶消化样本中的加合物进行定量。铸铁厂工人的白蛋白加合物水平（5.22飞摩尔/微克，0.314毫摩尔/摩尔）高于参照组（4.07飞摩尔/微克，0.245毫摩尔/摩尔），但可能由于样本量小，仅具有临界显著性。在一部分铸铁厂工人中，观察到休假后立即采集的样本与工作场所暴露六周后的样本在加合物水平上存在显著差异。屋顶工的加合物水平（5.19飞摩尔/微克，0.312毫摩尔/摩尔）也高于其参照组（3.28飞摩尔/微克，0.197毫摩尔/摩尔）。这些结果证明了PAH蛋白加合物测量作为人类接触此类化学物质标志物方法的可行性。
