Santella R M, Hemminki K, Tang D L, Paik M, Ottman R, Young T L, Savela K, Vodickova L, Dickey C, Whyatt R
Division of Environmental Science, School of Public Health, Columbia University, New York, New York 10032.
Cancer Epidemiol Biomarkers Prev. 1993 Jan-Feb;2(1):59-62.
In an ongoing comprehensive evaluation of biological markers, workers in or near an iron foundry with varying exposure to polycyclic aromatic hydrocarbons (PAH) were analyzed for molecular response to this exposure. Exposure to benzo(a)pyrene, determined by personal monitors worn by the workers (2 to 60 ng/m3), was considerably lower than in a previous study at this foundry (< 50 to 200 ng/m3) (F.P. Perera et al., Cancer Res., 48: 2288-2291, 1988). Two biomarkers, 1-hydroxypyrene in urine measured by high-performance liquid chromatography with fluorescence detection (a measure of internal dose) and PAH-DNA adducts in WBC measured by immunoassay (a measure of biologically effective dose) were assessed to demonstrate their relationship to the lowest exposures yet analyzed in foundry workers. In addition, these markers were analyzed for dose response and interindividual variability. Cigarette smoking, but not age or charbroiled food, influenced the level of 1-hydroxypyrene but not PAH-DNA adducts. When workers were classified into three exposure categories (low, medium, and high), mean 1-hydroxypyrene levels were 2.7, 1.8, and 3.6 mumol/mol creatinine, respectively. Comparisons by analysis of variance showed a significant difference between the groups after controlling for smoking (P = 0.02), but a trend test using multivariate linear regression analysis was not significant (r = 0.27; P = 0.07). Substantial interindividual variation was demonstrated by the 19- to 20-fold range in the values within each of the three exposure groups.(ABSTRACT TRUNCATED AT 250 WORDS)
在一项正在进行的生物标志物综合评估中，对不同程度接触多环芳烃（PAH）的铸铁厂内或附近的工人进行了分析，以研究其对这种接触的分子反应。通过工人佩戴的个人监测器测定的苯并(a)芘接触量（2至60 ng/m3），远低于该铸铁厂此前一项研究中的接触量（<50至200 ng/m3）（F.P.佩雷拉等人，《癌症研究》，48: 2288 - 2291, 1988）。评估了两种生物标志物，一种是通过高效液相色谱荧光检测法测定的尿中1 - 羟基芘（衡量体内剂量），另一种是通过免疫测定法测定的白细胞中PAH - DNA加合物（衡量生物有效剂量），以证明它们与铸铁厂工人中迄今分析的最低接触量之间的关系。此外，还分析了这些标志物的剂量反应和个体间差异。吸烟会影响1 - 羟基芘的水平，但年龄和烧烤食物不会，吸烟对PAH - DNA加合物水平没有影响。当将工人分为三个接触类别（低、中、高）时，平均1 - 羟基芘水平分别为2.7、1.8和3.6 μmol/mol肌酐。方差分析比较显示，在控制吸烟因素后，各组之间存在显著差异（P = 0.02），但使用多元线性回归分析的趋势检验不显著（r = 0.27；P = 0.07）。三个接触组中每组数值的范围为19至20倍，表明个体间存在很大差异。（摘要截短为250字）
