高龄对经空气感染结核分枝杆菌的豚鼠接种卡介苗的影响。

Influence of advanced age on Mycobacterium bovis BCG vaccination in guinea pigs aerogenically infected with Mycobacterium tuberculosis.

作者信息

Komine-Aizawa Shihoko, Yamazaki Toshio, Yamazaki Tsuyoshi, Hattori Shin-Ichiro, Miyamoto Yuji, Yamamoto Naoki, Haga Shinji, Sugitani Masahiko, Honda Mitsuo, Hayakawa Satoshi, Yamamoto Saburo

机构信息

Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabash-ku, Tokyo, Japan.

出版信息

Clin Vaccine Immunol. 2010 Oct;17(10):1500-6. doi: 10.1128/CVI.00190-10. Epub 2010 Aug 4.

DOI:10.1128/CVI.00190-10

PMID:20685935

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2953003/

Abstract

Mycobacterium bovis bacillus Calmette-Guérin (BCG) is the only tuberculosis (TB) vaccine currently available, but its efficacy against adult pulmonary TB remains controversial. BCG induces specific immune responses to mycobacterial antigens and may elicit protective immunity against TB. TB remains a major public health problem, especially among the elderly, yet the efficacy of BCG in the elderly is unknown. We investigated the ability of BCG vaccination to prevent TB in young (6-week-old), middle-aged (18-month-old), and old (60-month-old) guinea pigs. BCG-Tokyo vaccination reduced the growth of Mycobacterium tuberculosis H37Rv in all three groups. By use of an enzyme-linked immunospot (ELISPOT) assay, antigen-specific gamma interferon (IFN-γ)-producing cells were detected in the 60-month-old guinea pigs after a booster vaccination with BCG-Tokyo. Our findings suggest that BCG-Tokyo has a protective effect against tuberculosis infection regardless of age.

摘要

牛分枝杆菌卡介苗（BCG）是目前唯一可用的结核病疫苗，但其对成人肺结核的疗效仍存在争议。卡介苗可诱导针对分枝杆菌抗原的特异性免疫反应，并可能引发针对结核病的保护性免疫。结核病仍然是一个主要的公共卫生问题，尤其是在老年人中，然而卡介苗在老年人中的疗效尚不清楚。我们研究了卡介苗接种对年轻（6周龄）、中年（18月龄）和老年（60月龄）豚鼠预防结核病的能力。东京卡介苗接种减少了所有三组中结核分枝杆菌H37Rv的生长。通过酶联免疫斑点（ELISPOT）分析，在用东京卡介苗加强免疫后，在60月龄的豚鼠中检测到了产生抗原特异性γ干扰素（IFN-γ）的细胞。我们的研究结果表明，无论年龄大小，东京卡介苗对结核感染都有保护作用。