识别一种非荚膜抗原的鼠单克隆抗体，该抗原可区分新生隐球菌新生变种和新生隐球菌格特变种。

Murine monoclonal antibodies recognizing a non-capsular antigen that distinguishes between Cryptococcus neoformans var. neoformans and C. neoformans var. gattii.

作者信息

Hamilton A J, Bartholomew M A, Figueroa J, Fenelon L E, Hay R J

机构信息

Dermatology Unit, Department of Clinical Sciences, Guy's Hospital, London Bridge, UK.

出版信息

Trans R Soc Trop Med Hyg. 1991 Jan-Feb;85(1):123-7. doi: 10.1016/0035-9203(91)90184-z.

DOI:10.1016/0035-9203(91)90184-z

PMID:2068741

Abstract

A panel of 4 monoclonal antibodies (mabs) of the IgG1 subclass have been made against a cytoplasmic antigen of Cryptococcus neoformans. Mab 4E2 recognized isolates of C. neoformans var. gatti by enzyme-linked immunosorbent assay (ELISA), whilst the other antibodies did not recognize these antigens. By Western blot 4E2 recognized determinants at 110-125, 65-70, 45-50 and 36-38 kDa. Mabs 9E6, 7C7 and 5D9 recognized bands at 36-38 and approximately 30 kDa. All 4 mabs (4E2, 9E6, 7C7 and 5D9) recognized both non-encapsulated and encapsulated isolates of C. neoformans var. neoformans by ELISA, and in addition showed reactivity to only the cytoplasm and cell membrane of yeasts by immunofluorescence. Mab 7C7 recognized antigens of the closely related fungus Trichosporon beigelii by ELISA but did not recognize any other fungal antigens. The other 3 mabs showed no recognition of T. beigelii or any other fungal pathogens tested.

摘要

已制备出一组针对新型隐球菌胞质抗原的4种IgG1亚类单克隆抗体（mab）。单克隆抗体4E2通过酶联免疫吸附测定（ELISA）识别新型隐球菌变种加蒂的分离株，而其他抗体不能识别这些抗原。通过蛋白质印迹法，4E2识别出分子量为110 - 125 kDa、65 - 70 kDa、45 - 50 kDa和36 - 38 kDa的决定簇。单克隆抗体9E6、7C7和5D9识别出分子量为36 - 38 kDa和约30 kDa的条带。所有4种单克隆抗体（4E2、9E6、7C7和5D9）通过ELISA识别新型隐球菌变种新型隐球菌的非荚膜和荚膜分离株，此外，通过免疫荧光显示仅对酵母的细胞质和细胞膜有反应性。单克隆抗体7C7通过ELISA识别密切相关的真菌白吉利丝孢酵母的抗原，但不识别任何其他真菌抗原。其他3种单克隆抗体未识别白吉利丝孢酵母或任何其他测试的真菌病原体。