用于新型隐球菌多糖抗原的夹心酶联免疫吸附测定的灵敏度取决于捕获抗体和检测抗体的同种型。
Sensitivity of sandwich enzyme-linked immunosorbent assay for Cryptococcus neoformans polysaccharide antigen is dependent on the isotypes of the capture and detection antibodies.
作者信息
Mukherjee S, Casadevall A
机构信息
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
出版信息
J Clin Microbiol. 1995 Mar;33(3):765-8. doi: 10.1128/jcm.33.3.765-768.1995.
Abstract
Immunoglobulin M (IgM) and IgG1 monoclonal antibody isotype switch variants to Cryptococcus neoformans capsular polysaccharide were used to study the sensitivity of a double sandwich enzyme-linked immunosorbent assay (ELISA). The most sensitive ELISA configurations used IgG1 monoclonal antibody absorbed on polystyrene plates or IgM immobilized with goat antisera for antigen capture.
摘要
免疫球蛋白M（IgM）和IgG1单克隆抗体同型转换变体针对新型隐球菌荚膜多糖，用于研究双夹心酶联免疫吸附测定（ELISA）的敏感性。最敏感的ELISA配置使用吸附在聚苯乙烯板上的IgG1单克隆抗体或用山羊抗血清固定的IgM进行抗原捕获。
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