Trask B J
Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA 94550.
Trends Genet. 1991 May;7(5):149-54. doi: 10.1016/0168-9525(91)90378-4.
Unique sequences, chromosomal subregions, or entire genomes can be specifically highlighted in metaphase or interphase cells by fluorescence in situ hybridization (FISH). This technique can be used to identify chromosomes, detect chromosomal abnormalities or determine the chromosomal location of specific sequences. FISH plays an increasingly important role in a variety of research areas, including cytogenetics, prenatal diagnosis, tumor biology, gene amplification and gene mapping.
通过荧光原位杂交（FISH）技术，独特序列、染色体亚区域或整个基因组可在中期或间期细胞中被特异性标记。该技术可用于识别染色体、检测染色体异常或确定特定序列的染色体定位。FISH在包括细胞遗传学、产前诊断、肿瘤生物学、基因扩增和基因图谱绘制等多种研究领域发挥着越来越重要的作用。
