Appelt G, Hänsler M, Rogos R
Forschungsabteilung Gastroenterologie, Bereichs Medizin, Universität Leipzig.
Z Med Lab Diagn. 1991;32(2):72-8.
Thin layer urea isoelectric focusing is a powerful tool for a rapid and reproducible high resolution separation of cholecystokinin stimulated human pancreatic proteins. In pancreatic juice of patients without pancreatic disease, 11 main protein bands were separated and distinguished by Coomassie Blue-R 250 staining. A number of faint protein bands divided among the whole pH range demonstrate a wide microheterogeneity of exocrine pancreatic proteins. Secretory protein patterns of patients with chronic pancreatitis are shown to be different from those of patients without pancreatic disease. Significant alterations were found at the acidic protein band region of pI 4.4 to 4.7. In two cases of chronic pancreatitis the IEF pattern was very similar to that obtained by a short time enteropeptidase incubation of pure pancreatic juice from patients without pancreatic disease.
薄层尿素等电聚焦是一种用于快速、可重复且高分辨率分离胆囊收缩素刺激的人胰腺蛋白的强大工具。在无胰腺疾病患者的胰液中，通过考马斯亮蓝 - R 250染色分离并区分出11条主要蛋白带。在整个pH范围内分布的一些微弱蛋白带表明外分泌胰腺蛋白具有广泛的微观异质性。慢性胰腺炎患者的分泌蛋白模式显示与无胰腺疾病患者不同。在等电点4.4至4.7的酸性蛋白带区域发现了显著变化。在两例慢性胰腺炎病例中，等电聚焦模式与对无胰腺疾病患者的纯胰液进行短时间肠肽酶孵育所获得的模式非常相似。
