Appelt G, Schulze B, Rogos R, Kopperschläger G
Institute of Policlinical Medicine, Karl Marx University Leipzig, GDR.
Biomed Biochim Acta. 1988;47(2):133-40.
Pancreatic juice collected by endoscopic retrograde cannulation of the pancreatic duct after administration of secretin and pancreozymin was analyzed by pore gradient polyacrylamide gel electrophoresis. Two electrophoretic systems, an alkaline (pH 8.9) and an acidic (pH 5.0), were elaborated to obtain optimal separation of exocrine proteins. 13 bands under alkaline conditions and 17 bands by running in the acidic system were separated and distinguished by Coomassie-Brilliant Blue G 250 staining. Fourteen proteins were identified using enzymatic and immunological methods: one form of each of lipase, chymotrypsinogen, prophospholipase A2, procarboxypeptidase A and trypsin inhibitor, two forms of each of procarboxypeptidase B and proelastase, three forms of each of trypsinogen and amylase. An additional band was identified as albumin.
在注射促胰液素和缩胆囊素后，通过内镜逆行胰管插管收集胰液，并采用孔径梯度聚丙烯酰胺凝胶电泳进行分析。为了实现外分泌蛋白的最佳分离，构建了两种电泳系统，一种是碱性（pH 8.9）系统，另一种是酸性（pH 5.0）系统。在碱性条件下分离出13条带，在酸性系统中电泳分离出17条带，并用考马斯亮蓝G 250染色进行区分。使用酶法和免疫法鉴定出14种蛋白质：脂肪酶、胰凝乳蛋白酶原、前磷脂酶A2、前羧肽酶A和胰蛋白酶抑制剂各一种形式，前羧肽酶B和前弹性蛋白酶各两种形式，胰蛋白酶原和淀粉酶各三种形式。另外一条带被鉴定为白蛋白。
