Araújo João Pessoa, Nogueira Márcia F, Duarte José M B
Instituto de Biociências, Universidade Estadual Paulista (UNESP), Departamento de Microbiologia e Imunologia, 18618-000, Botucatu, São Paulo, Brazil.
J Wildl Dis. 2010 Jul;46(3):939-43. doi: 10.7589/0090-3558-46.3.939.
Habitat fragmentation and diseases have resulted in a decline of the marsh deer (Blastocerus dichotomus) throughout its South American range. Our objectives were to determine whether marsh deer intended for translocation from a region of the Rio Paraná Basin had been infected previously by foot-and-mouth disease virus (FMDV) and whether they were carrying virus. We captured marsh deer from June to October 1998 and collected blood from 108 animals and esophageal-pharyngeal fluid from 53. Serum was tested for antibodies against three FMDV serotypes (O, A, and C) by liquid-phase-blocking sandwich enzyme-linked immunosorbent assay (ELISA). Esophageal-pharyngeal fluid was tested for FMDV RNA by reverse transcription polymerase chain reaction (RT-PCR) and inoculation into three successive baby hamster kidney (BHK-21) cell subcultures, followed by RT-PCR of cultures. We detected low log(10) titers (range 1.0-1.5) to FMDV subtype A(24) Cruzeiro in 19 of 108 sampled marsh deer, but failed to isolate FMDV or detect FMDV RNA in any samples. We conclude that marsh deer from our study site were unlikely to carry FMDV; however, as a preventive measure, the 19 animals with titers for FMDV were not sent to FMDV-free Brazilian states.
栖息地破碎化和疾病导致南美泽鹿（Blastocerus dichotomus）在其整个南美分布范围内数量减少。我们的目标是确定拟从巴拉那河流域一个地区进行易地放归的南美泽鹿此前是否感染过口蹄疫病毒（FMDV）以及它们是否携带该病毒。我们于1998年6月至10月捕获南美泽鹿，从108只动物采集血液，从53只采集食管 - 咽液。血清通过液相阻断夹心酶联免疫吸附测定（ELISA）检测针对三种口蹄疫病毒血清型（O、A和C）的抗体。食管 - 咽液通过逆转录聚合酶链反应（RT-PCR）检测口蹄疫病毒RNA，并接种到三代连续传代的幼仓鼠肾（BHK-21）细胞培养物中，随后对培养物进行RT-PCR检测。我们在108只采样的南美泽鹿中的19只中检测到对口蹄疫病毒A（24）亚型克鲁塞罗毒株的低log(10)滴度（范围为1.0 - 1.5），但在任何样本中均未分离到口蹄疫病毒或检测到口蹄疫病毒RNA。我们得出结论，来自我们研究地点的南美泽鹿不太可能携带口蹄疫病毒；然而，作为一项预防措施，这19只具有口蹄疫病毒滴度的动物未被送往无口蹄疫病毒的巴西各州。
